气相色谱内标法检测烟碱含量方法学考察.doc

生物与化学工程学院 仪器分析实训报告 题目：气相色谱内标法检测烟碱含量方法学考察 Study on the methodology investigation of detecting nicotine content by GC internal standard method 专业：食品科学与工程 姓名： 戚效群 学号：10501094002 完成日期：2010-5-24 气相色谱内标法检测烟碱含量方法学考察 【摘 要】烟碱(又称尼古丁)是烟叶中的主要成分。本次试验以喹啉为内标物，烟碱为测定对象，考查及检测气相色谱法内标法测定烟碱含量的方法学。试验中以有机溶剂萃取、填充气相色谱法测定对试验的重现性及精密度和线性关系进行进行了认真分析。一定范围内，烟碱含量的峰高比及面积比呈现线性关系，重现性及精密度良好。 【关键词】： 内标法 烟碱 气相色谱 方法学 Study on the methodology investigation of detecting nicotine content by GC internal standard method Abstract：Nicotine (also called nicotine) is the main ingredient. Tobacco This trial as internal standard with things, -1h-qunolin-2-ones nicotine to determine object, examines and detecting gas chromatography internal standard method for determining the content of nornicotine methodology. Experiments with organic solvent extraction, filling gas chromatography was developed for the determination of the test the reproducibility and precision and linear relationship between analyzed carefully. A certain range, high ratio of nicotine content than present peak area of linear relation, reproducibility and precision is good. Keywords： Internal standard method nicotine gas chromatography methodology 目 录 1 绪论……………………………………………………………………5 1.1 烟碱的结构与化学性质………………………………………………5 1.2 烟碱的检测方法………………………………………………………6 1.2.1 非水滴定法检测烟碱含量…………………………………………6 1.2.2 紫外线分光光度法检测烟碱含量…………………………………6 1.2.3 水蒸气蒸馏法检测烟碱含量………………………………………6 1.2.4 高效液相色谱法检测烟碱含量……………………………………7 1.2.5 气相色谱法检测烟碱含量…………………………………………7 1.2.5.1 外标法检测烟碱含量……………………………………………8 1.2.5.2 归一化法检测烟碱含量…………………………………………8 1.2.5.3 内标法检测烟碱含量……………………………………………8 1.3 检测方法学考察……………………………………………………9 1.3.1 线性关系的考察……………………………………………………9 1.3.2 精密度的考察………………………………………………………9 1.3.3 重现性实验的考察…………………………………………………10 1.3.4 回收率………………………………………………………………10 1.3.5 验证性………………………………………………………………10 2 实验部分………