NP对SBR系统中典型微生物菌群的影响.docVIP

NP对SBR系统中典型微生物菌群的影响摘 要壬基酚(Nonlphenol, NP) 是一种对生物有机体具有毒害作用的内分泌干扰物( endocrine disrupting chemicals, EDCs)，主要用于生产表面活性剂壬基酚聚氧乙烯醚(NPmEO)。环境中的壬基酚主要来源于污水处理厂系统中NPmEO的不完全降解后的出水中，并且大量野生动物与实验动物研究证明了壬基酚具有雌激素效应和其他的生物毒性, 其对生物体产生的不良作用包括影响内分泌、影响生殖和发育、影响免疫及促癌作用等。壬基酚被认为是有代表性的环境内分泌干扰物, 为联合国环境保护署制订的27 种优先控制的持久性有毒污染物之一。尽管一些研究对壬基酚在污水处理系统中的迁移转化进行了探讨，但是通过工业废水和生活污水排放进入污水处理系统中的壬基酚是否会对污水处理系统中的微生物种群及污泥活性产生影响尚不明确。因此本论文通过投加不同浓度梯度的壬基酚到经人工配置生活污水驯化的活性污泥中，进行摇瓶实验并进行分析，探索壬基酚对活性污泥可培养微生物的影响关键词：壬基酚；活性污泥；SBR；菌群；酶活性； Effect of NP on typical microorganisms in the SBR systemAbstractNonylphenol (NP) is an endocrine disrupting chemicals(EDCs) with the toxic effect for a biological organism, and in actual production, nonylphenol mainly uses for the production of surfactant nonylphenol ethoxylate (NPmEO). In the environment, nonylphenol mainly produced from incomplete degradation of NPmEO in the sewage treatment plant Nonylphenol system. And according to studies on a large number of wild and laboratory animals, the results show that nonylphenol has estrogenic effects and other toxicity, the adverse effects on organisms of which include impacts of endocrine, reproduction and development, immune and cancer promoting. Nonylphenol is considered as a representative of environmental endocrine disruptors and it was listed as one of the 27 priority persistent toxic pollutants by Environmental Protection Department for the United Nations. Although the migration and transformation of nonylphenol in sewage treatment systems has been discussed in some studies, it is still unclear whether nonylphenol has effects on microbial population and sludge activity which is discharged into the sewage treatment system through industrial wastewater and domestic sewage. Therefore, in this thesis, we made shake flask experiments through adding different concentrations of nonylphenol to manually configured activated sludge acclimated by domestic sewage to explore the culture of activated sludge microorganisms and the effects of typical en