1陈泽斌,夏振远,雷丽萍,陈海如.防治烟草黑胫病有益内生细菌的筛选.docVIP

烟草黑胫病生防菌的筛选、定殖及应用评价 曾衡1 徐迪红1,3 冀志霞1 陈守文1 魏雪团1,2** （1华中农业大学农业微生物学国家重点实验室 武汉 430070） （2华中农业大学食品科技学院 武汉 430070） （3华中农业大学工学院 武汉 430070） 摘要 烟草黑胫病是一种危害严重的土传真菌性病害，对我国烟草种植业造成了巨大的经济损失。为有效防治烟草黑胫病，本研究筛选了三株可高效拮抗烟草黑胫病菌（Phytophthora parasitica var . nicotianae）的生防菌ZH、C9和C20，经生理生化和基因序列分析，三株菌都被鉴定为解淀粉芽胞杆菌。以ZH为代表性菌株进行定殖实验，结果显示，解淀粉芽胞杆菌ZH在土壤和烟草植株体内都具有良好的定殖能力，123 d后根际土壤和根围土壤中生防菌的生物量分别达到9.07×106 CFU/g和1.2×107 CFU/g，烟草茎、叶分别达到1.83×104 CFU/g和5.58×104 CFU/g。以正常施用化学药剂的烟草为对照，以ZH、C9和C20混合菌剂进行生防菌大田应用试验。与对照相比，施用混合菌株发酵液不同时期的防效为85.37%－95.11%。同时，烟草的产量相比对照每亩增产20.18%，每亩增值31.27%。本研究提供了一种新型高效的烟草黑胫病生防菌及其大田应用方法，具有重要的应用价值。 关键词：烟草黑胫病；生物防治；解淀粉芽胞杆菌；定殖 Isolation of antagonistic bacteria against tobacco black shank disease and evaluation of the colonization and bio-control ability ZENG Heng1, XU Dihong1,3, JI Zhixia1, CHEN Shouwen1 WEI Xuetuan1,2** 1State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China 2College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China 3Institute of technology, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China Abstract Tobacco black shank disease is a serious soil-spread fungal disease, leading to great economic loss to tobacco crop of China. To prevent and control of tobacco black shank disease, three strains (ZH, C9 and C20) with high antagonist ability against tobacco black shank pathogens (Phytophthora parasitica var. Nicotianae) were isolated. By physiological, biochemical test and gene sequence analysis, these three strains were identified as Bacillus amyloliquefaciens. Colonization experimental results showed that the B. amyloliquefaciens ZH had high colonization ability in the plant and soil. After 123 d, the bio-control bacteria number in the rhizosphere soil and rhizosphere soil reached 9.07 x 106 CFU/g and 1.2 x 107 CFU/g, respectively, as well as high cell number in tobacco stem (1.83 x 104 CFU/g) and leaf (5.58