DNA in My Food Biotechnology我的食品生物技术.pptVIP

DNA in My Food??? DNA in My Food??? DNA is present in the cells of all living organisms. The process of extracting DNA from a cell is the first step for many laboratory procedures in biotechnology. The scientist must be able to separate DNA from the unwanted substances of the cell gently enough so that the DNA does not denature (break up). DNA in My Food??? You will prepare a solution of banana treated with salt, distilled water, and shampoo (detergent). The detergent breaks down the cell membrane by dissolving the lipids (fatty molecules) and proteins of the cell and disrupting the bonds that hold the cell membrane together. DNA in My Food??? The detergent then forms complexes with these lipids and proteins, allowing them to be filtered out of solution by the coffee filter. The cells’ DNA is left in the filtrate. The salt allows the DNA to precipitate out of filtrate in cold alcohol solution (Mg and Cl ions). DNA in My Food??? DNA in My Food??? DNA in My Food???Step 1 In a blender, mix a ratio of one banana per one cup (250ml) of distilled water. DNA in My Food???Step 1 (cont.) Blend for 15-20 seconds, until the solution is a mixture. DNA in My Food??? Step 2 In one of the 5 oz cups, make a solution consisting of 1 teaspoon of shampoo and two pinches of table salt. DNA in My Food??? Step 2 (cont.) Add 20 ml (4 teaspoons) of distilled water or until the cup is 1/3 full. Dissolve the salt and shampoo by stirring slowly with the plastic spoon to avoid foaming. DNA in My Food??? Step 3 To the solution you made in step 2, add three heaping teaspoons of the banana mixture from step 1. Mix the solution with the spoon for 5-10 minutes. DNA in My Food??? Step 4 While one member of your group mixes the banana solution, another member will place a #2 cone coffee filter inside the second 5 oz plastic cup. Fold the coffee filter’s edge around the cup so that the filter does not touch the bottom of the cup. DNA in My Food??? Step 5 Filter the mixture by pour