晚期氧化蛋白产物对小鼠腹腔巨噬细胞产生一氧化氮的影响.docVIP

晚期氧化蛋白产物对小鼠腹腔巨噬细胞产生一氧化氮的影响 作者：李忠海；刘尚喜；侯凡凡；王燕群　　(南方医科大学南方医院肾内科，广东??广州??510515) 摘要：目的??研究晚期氧化蛋白产物(AOPP)对小鼠腹腔巨噬细胞(MPM)产生一氧化氮的影响。方法??用次氯酸氧化牛血清白蛋白(BSA)制备的AOPP-BSA 单独或与内毒素(LPS)一起与MPM共同培养或用AOPP-BSA预处理后再用LPS刺激MPM,用Griess试剂法测定培养上清一氧化氮的含量；MTT法测定细胞活力。结果??AOPP修饰可使BSA诱导MPM产生一氧化氮的量显著性降低。同时，AOPP-BSA预处理或与LPS一起共同刺激巨噬细胞均可显著性抑制LPS诱导的MPM产生一氧化氮,抑制程度与BSA的氧化修饰程度AOPP-BSA的浓度有关。结论??晚期氧化蛋白产物可抑制MPM诱导型一氧化氮的产生。 关键词：晚期氧化蛋白产物；氧化应激；巨噬细胞；一氧化氮 中图分类号：Q251??文献标识码：A??文章编号：1673-4254(2006)05-0558-03 Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophagesLI Zhong-hai； LIU SHANG-Xi； HOU Fan-fan； WANG Yan-qunDePartment of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, ChinaAbstract: Objective??To investigate the effect of advanced oxidation protein products (AOPP) on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs). Methods??MPMs were incubated in the absence or presence of lipopolysaccharide (LPS) with AOPP-modified bovine serum albumin (BSA) prePared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS. NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method. The cell viability was measured by MTT assay. Results??BSA induced significant NO production in MPMs. AOPP modification of BSA significantly inhibited NO production, and AOPP-BSA exhibited time- and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation. Conclusion??AOPP-BSA is caPable of inhibiting inducible NO production in MPMs.Key words: advanced oxidation protein products; oxidative stress; macrophages; nitric oxide收稿日期：2005-10-24基金项目：国家自然科学基金重点项目、面上项目Supported by National Natural Science Foundation of China作者简介：李忠海(1981-)，男，在读硕士研究生，电话：020E-mail:loy5460@通讯作者：刘尚喜，电话E-mail:liusx@ ????终末期肾脏病(ESR