癫痫大鼠海马线粒体COXⅠ和COXⅣ表达的变化.docVIP

癫痫大鼠海马线粒体COXⅠ和COXⅣ表达的变化 　　作者：王小花 高静 张同霞 赵秀鹤 刘学伍 迟兆富 作者单位：山东大学齐鲁医院神经内科，山东 济南 250012 　　【摘要】 目的 探讨癫痫对大鼠海马线粒体细胞色素氧化酶(COX)的mtDNA和nDNA编码亚基Ⅰ、Ⅳ表达的影响。方法 成年雄性Wistar大鼠随机分为生理盐水对照组、癫痫持续状态(SE)后急性期(3 h)、静止期(7 d)、慢性期(45 d)组和注射匹鲁卡品(PILO)但未出现SE组。免疫组化并荧光实时定量PCR检测海马线粒体COXⅠ、Ⅳ阳性染色及其mRNA的表达。结果 COXⅠ阳性细胞数在SE后3 h明显增加，7 d降到对照组水平，45 d显著降低;COX Ⅳ阳性细胞数SE后3 h稍有增加，但较对照组无显著性差异，7 和45 d时COXⅣ染色变化与对照组相似;注射PILO但未出现SE组COXⅠ、Ⅳ的表达与对照组类似。COXⅠmRNA在SE后3 h表达明显升高(P0.001)，7 d时降到对照水平，45 d显著降低(P0.001);COXⅣ mRNA在3 h时表达较对照组升高，但差异无显著性，7 和45 d时下降至对照组水平。注射PILO但无SE组与对照组结果类似。结论 颞叶癫痫海马COX功能紊乱与痫性发作相关，线粒体编码的基因更易受痫性发作的影响。 　　【关键词】 细胞色素氧化酶;癫痫;海马;线粒体 　　基金项目：山东省十一五重大攻关计划(2005gg3202069) 　　Changes of expression of mitochondrial cytochrome oxidase subunits Ⅰ and Ⅳ in the hippocampus of pilocarpine-treated rats 　　WANG Xiao-Hua，GAO Jing，ZHANG Tong-Xia,et al. 　　Department of Neurology，Qilu Hospital of Shandong University，Jinan 250012，Shandong，China 　　【Abstract】 Objective To investigate the effects of epilepsy on expression of cytochrome oxidase(COX) subnuitsⅠ(COXⅠ) and Ⅳ(COX Ⅳ) encoded by mtDNA and nDNA respectively in rat hippocampus.Methods Male Wistar rats were divided randomly into saline control, acute period(3 h),silent period(7 d),chronic period groups(45 d) after status epilepticus(SE) and group which received pilocarpine (PILO) but did not develop SE.The expressions of COXⅠ and COX Ⅳ in rat hippocampus were detected respectively by immunohistochemical staining and real time quantitative PCR.Results An significant increase in COXⅠ staining was observed in neuronal cell bodies distributed throughout the hippocampus(CA1，CA2，CA3，dentate gyrus) of rats killed 3 h after SE，when compared to the saline-treated group on day 7 after SE，the immunoreactivity was slowly reduced and the immunoreactivity of cell bodies was restricted predominantly to the dentate gyrus，and CA3 regions of the hippocampus.The chronic period (45 d) showed decreased staining with the findings indicating neuronal degeneration such as