summerstudentreport.pptVIP

SUMMER STUDENT REPORT Project I:Mouse behavior Project II:Mouse brain development rotarod Major select mutants by its balance ability. We control rotating speed and acceleration time Result as follows 10504 10505 lack balance ability. 10485 good balance ability. 10504 is short of activity and curiosity. 10485 10459 have high activity and courage Next step Thanks * * 葉明憲 National Tsing Hua University Life Science 05 Dr. Ying-Hue Lee Institute of Molecular Biology Academia Sinica R.O.C Date:2003/7/1~8/29 Purpose: find special mutants Material:F3 EMS mouse Selection type Rota rod Hole board Open-field Water maze Result : Project I mouse behavior 60sec 10rpm 1rpm practice 60sec 60rpm 1rpm Trail C 1sec 20rpm 1rpm Trail B 60sec 20rpm 1rpm Trail A Acceleration time Final speed Initial speed Data analysis Trail A 1 to 20 rpm /60sec Average=18.29rpm 16 mice up to 15rpm It’s a easy job for mice. Still we can select the worst in balance ability. Trail B 1 to 20 rpm/ 1sec Data analysis High-speed motor coordination The curve represent the division into two opposing extremes ?success or failure Data analysis Trail C 1 to 60 rpm/60sec Select the best and the worst Approach normal distribution. Rotarod result excellent worst 32.48 100.95 18.29 102.89 average 58.12 78.02 20 132.27 10486 53.228 54.2 20 136.62 10485 9.04 2.51 11.57 39.15 10505 9.32 3.52 10.58 33.45 10504 C rpm B sec A rpm A sec back Hole board A board has 4 X 4 holes arranged square in a plane Making the lighting dull enable the mice to take a look into the hole,and dark light make the mice Comfortable and in a leisurely manner. We select by it curiosity and activity. Data analysis The two trail correlation coefficient=0.405 The data we obtain regard as activity reference. This result also could be compared with open-field It should be more trails. 10496 have lowest frequency to look into the hole Almost he just pass the hole and look the w