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Real-time quantitative PCR (qPCR) analysis
TATAA Genomics Core Facility offers you a broad selection of real-time PCR instruments, including fast cyclers, High Resolution Melt (HRM) cyclers, and high throughput instruments. Applications such as gene expression analysis, genotyping, SNP analysis, HRM, digital PCR, Immuno-qPCR, and any more esoteric application of your choice are easily performed with confidence. TATAA’s experts can also advice on how to select the optimum reference genes or perhaps use alternative means to normalize your data.
Examples of real-time PCR analyses that can be performed at TATAA Genomics Core Facility are:
Gene expression analysis (relative and absolute quantification)
Genotyping
Copy number variation (CNV) analysis
miRNA analysis
DNA methylation analysis
CpG-methylation analysis
SNP typing
Allelic discrimination (end-point analysis)
Digital PCR
Protein quantification with immuno qPCR
qPCR data analysis and data mining with access to user friendly professional softwares
or other applications of your choice
Our G?teborg facility’s real-time PCR instrument park is the most advanced in Europe. We have instruments from all leading manufacturers, several of which are capable of High Resolution Melt (HRM) analysis. You can pipette and setup reactions manually or use our automated liquid handling systems to save time and standardize procedures.
For your convenience plastic consumables such as plates, strips, tubes, pipette tips etc. for your experiments are included in our services. All have been tested for best performance by our experts, and all instruments are regularly calibrated with those consumables to ascertain optimum performance and highest possible repeatability and reproducibility. We are also carefully selecting real-time PCR reagents for optimum performance and lowest cost, available for you to purchase. For work in other labs, you can purchase the consumables from us separately to ascertain you can use the same protocols and sti
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