IL-18在病理性瘢痕中的表达及其生物学意义.docVIP

IL-18在病理性瘢痕中的表达及其生物学意义 　　[摘要]目的：比较IL-18在正常皮肤和病理性瘢痕组织中的表达及分布情况，探讨IL-18在病理性瘢痕的形成过程中所起的生物学作用及意义。方法：收集2006年12月到2007年6月广东医学院附属医院整形外科手术切除的正常皮肤10例，增生性瘢痕组织12例，瘢痕疙瘩12例，应用免疫组织化学技术及荧光定量RT-PCR分别检测IL-18蛋白及IL-18 mRNA在它们中的表达水平及分布情况。结果：IL-18在正常皮肤、增生性瘢痕及瘢痕疙瘩中均有表达，IL-18mRNA和 IL-18蛋白在瘢痕疙瘩组和增生性瘢痕组之间无明显差异(P0.05)，但两者均低于正常皮肤组(P0.05)。结论：IL-18可能是病理性瘢痕组织的形成促进因素之一。 　　[关键词]IL-18；增生性瘢痕；瘢痕疙瘩；RT-PCR；免疫组织化学 　　[中图分类号]R619+.6 [文献标识码]A[文章编号]1008-6455（2014）06-0454-03 　　 　　The expression of IL-18 in pathological scars and its biological effect 　　WU Ze-yong,ZHANG Pei-hua,LI-Jin,LI Xiao-fang,YANG Chun-yan 　　（1.Department ofPlastic Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001,Guangdong,China; 2.Department ofPathology, Affiliated Hospital of Guangdong Medical College; 3.Surgery Laboratory of Clinical Skills Training Center,Guangdong Medical College） 　　 　　Abstract:ObjectiveComparing the expression and distribution of IL-18 in the normal skin and pathological scars, To explore the biological contribution of lL-18 in the formative course of hypertrophic scard.MethodsFrom December 2006 to June 2007，10 cases of normal skin, 12 cases of proliferative scar and 12 cases of keloid tissues were collected at Department of Plastic Surgery, Affiliated Hospital of Guangdong Medical College.Take normaI skin as control group,Applicating immunohistochemical technique and fluorescence quantitative RT-PCR, to detect the expression level and distribution of IL - 18 protein and IL - 18 mRNA in them. ResultsThe expression of IL-18 protein and IL-18 mRNA was both obviously lower in hypertrophic scar and keloid tissues compared with those of narmal skin respectively y(P0 05).ConcIusionThe results indicated that IL-18 maybe one of the factors to promote pathological scar tissue forming. 　　Key words:IL-18;hypertrophic scars;keloid;RT-PCR;immunohistochemical 　　病理性瘢痕为皮肤创伤后形成的过度增生性疾病，破坏人体表面完整性和组织器官正常结构，导致创伤部位畸形，功能障碍，影响美观，给患者带来极大的心理和经济负担。目前，其发病机制尚未完全清楚，一般认为是由创伤的迁延愈合，修复细胞中成纤维细胞的大量增殖与凋亡抑制，细胞外