低氧联合NaCl胁迫下外源Ca2+对发芽苦荞γ-氨基丁酸富集的.docVIP

低氧联合NaCl胁迫下外源Ca2+对发芽苦荞γ-氨基丁酸朱云辉郭元新( (安徽科技学院食品药品学院，安徽 凤阳 233100) 摘 要采用在通气的培养液中添加外源Ca2+培养的方式，研究了低氧联合NaCl胁迫下外源Ca2+对发芽苦荞GABA富集的影响。低氧联合NaCl胁迫对苦荞的芽长有抑制作用，GAD活力和GABA的含量随着NaCl浓度的增大均呈先上升后下降的趋势，在10 mmol/LNaCl溶液处理时均达到最高值，分别是单纯低氧胁迫的1.65和1.10倍；在低氧联合NaCl胁迫的基础上添加低浓度的Ca2+可缓解NaCl胁迫对苦荞芽长的抑制作用，添加3 mmol/L Ca2+处理可使GAD活力和GABA的富集量达到最大，分别为91.44 U/g FW和492.31 μg/g DW，分别是低氧联合NaCl胁迫的1.12倍和1.16倍；低氧联合NaCl胁迫下添加Ca2+螯合剂和通道阻断剂后发芽苦荞芽长显著降低，GAD活力显著下降，GABA富集量减少。外源Ca2+可降低低氧联合NaCl胁迫对苦荞芽长的抑制作用，并可通过提升GAD活力，增强发芽苦荞GABA的富集。 关键词低氧胁迫，NaCl胁迫，Ca2+处理，发芽苦荞，GABA富集中图分类号：TS201.1 文献标志码：A 文章编号： Exogenous Ca2+ on GABA Accumulation in Germinated Tartary Buckwheat under Hypoxia-NaCl Zhu Yunhui Guo Yuanxin Du Chuanlai Ding Zhigang Ye Hua (College of Food and Drug, Anhui Science and Technology University, Fengyang 233100) Abstract The effect of exogenous Ca2+ on GABA accumulation in germinated tartary buckwheat under hypoxia-NaCl stress was investigated by adding exogenous Ca2+ to the aeration medium of the aeration medium. The tartary buckwheat sprouts length were restrained under hypoxia-NaCl stress in which GAD activity and GABA content rose at first and then dropped with the increase of salt concentration. The maximum were reached at the 10 mmol/L salt solution, and were 1.65 times higher and 1.10 times higher than that under hypoxia stress alone respectively. On this basis, an addition of a low concentration of Ca2+ in hypoxia-NaCl stress could alleviate salt stress inhibition of the tartary buckwheat sprouts length. The addition of 3 mmol/L Ca2+ could make the GAD activity and GABA concentration reach the maximum, which were 91.44 U/g FW and 492.31 μg/g DW, 1.12 times higher and 1.16 times higher than that under hypoxia-NaCl stress respectively. Under hypoxia-NaCl stress, the germinated tartary buckwheat sprouts length decreased obviously, GAD activity was significantly decreased and GABA concentration decreased after the addition of Ca2+ chelating agent and channel blockers. Exogenous Ca2+ can relieve th