基于网络药理学的沙棘总黄酮治疗心肌缺血的作用机制研究.docxVIP

基于网络药理学的沙棘总黄酮治疗心肌缺血的作用机制研究魏志成1，童东1，杨娟1，赵可惠1，孟宪丽1，张艺1*(1.成都中医药大学，四川成都611137）[摘要]本研究采用网络药理学虚拟筛选方法，旨在进一步研究沙棘总黄酮（THF）治疗心肌缺血的活性成分和作用机制。首先通过TCMSP数据库和PubChem数据库，结合口服利用度和类药性分析，筛选出沙棘化合物中的黄酮类化合物。筛选得到的7个化合物再通过ChemMapper服务器进行靶点的预测分析。将得到的潜在靶点导入MAS 3.0数据库，结合KEGG数据库进行靶点分析和通路分析。最后使用Cystoscope 3.3.0软件绘制“化合物-靶点-作用通路”网络图。虚拟实验预测得到了68潜在靶点和60条信号通路，其中有31靶点和23条通路直接或间接与心肌缺血有关。结果表明，THF主要通过对钙离子信号通路（Calcium signaling pathway）、血管内皮生长因子信号通路（VEGF signaling pathway）和缝隙连接信号通路（Gap junction）等信号通路的调节发挥协同作用，且与文献报道吻合；提示其可能通过参与调节血小板聚集、脂质代谢、炎症反应等过程，增强心功能和对血管内皮细胞保护作用，从而发挥抗心肌缺血的作用。[关键词]沙棘；沙棘总黄酮；网络药理学；心肌缺血；机制研究Action mechanism of Hippophae rhamnoides in the treatment of myocardial ischemia based on network pharmacologyWEI Zhi-cheng1, YANG Juan1, MENG Xian-li1, ZHAO Ke-hui1, ZHANG Yi1*(1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China)[Abstract] In this study, a network pharmacological screening method to further study seabuckthorn flavonoids active ingredients and action mechanism for the treatment of myocardial ischemia.Firstly the use of ?the TCMSP database and PubChem database，combined with oral bioavailability and drug analysis，to screen flavonoids of Hippophae Rhamnoides compounds , then predictive analysis targets by ChemMapper server.Potential targets would be submitted to MAS 3.0. database and KEGG database to predict Potential targets and related pathway. Finally using Cystoscope 3.3.0 software rendering compounds - targets - pathway network diagram. Virtual experiments predicted 68 potential targets and 60 signaling pathway, which has 31 targets and 23 passages directly or indirectly associated with myocardial ischemia. The results showed that TFH mainly through the Calcium signaling pathway, VEGF signaling pathway and Gap junction signaling pathway regulates other related synergies, by suggesting that it may be involved in regulating platelet aggregation, lipid metabolism, inflammation and other processes, and enhance heart function Protective effects and vascular endoth