整合素连接激酶对大鼠离体心脏缺血再灌注室性-第三军医大学学报.docVIP

整合素连接激酶对大鼠离体心脏缺血再灌注室性心律失常的影响* 周平，蒋鑫，杨小利，曾春雨，方玉强 （第三军医大学大坪医院野战外科研究所心血管内科，重庆 400042） 摘要：目的：探讨整合素连接激酶(ILK)在大鼠离体心脏缺血再灌注室性心律失常中的作用。方法：采用Langendorff系统，结扎冠脉左前降支建立缺血再灌注心律失常模型，予ILK活性调节剂预处理，监测大鼠缺血再灌注室性心律失常的变化。结果：1. ILK激动剂呈剂量依赖的减少再灌注室性心律失常的发生，与缺血再灌注组（I/R）相比，中、高浓度组（10-7mol/L，2×10-7mol/L）恶性心律失常发生率及评分明显降低（P0.05）；2. ILK抑制剂呈剂量依赖的加重再灌注室性心律失常的发生，中、高浓度组（6×10-7mol/L，1.2×10-6mol/L）室性心律失常发生率及评分较I/R组明显增加（P0.05）。结论：ILK参与大鼠缺血再灌注室性心律失常的发生，调节其活性可能成为再灌注室性心律失常的治疗手段。 关键词：整合素连接激酶；缺血再灌注；室性心律失常 The effect of ILK on ventricular arrhythmia of rat ischemia reperfusion hearts Zhou Ping, Jiang Xin, Zeng Chunyu, Fang Yuqiang (Department of Cardiology, Daping Hospital,the Third Military Medical University,Chongqing 400042,China) Abstract Objective To explore the role of ILK in ventricular arrhythmia after ischemia-reperfusion(I/R) in isolated hearts. Methods Langendoff perfused rat hearts were subjected to 30 min of regional ischemia which induced by deligating the left anterior descending coronary artery, then 30 min of reperfusion. Surface ECG was used to observe arrhythmia continuously after pretreatment with regulator of ILK activity. Results The agonist and inhibitor of ILK could relieve or aggravate ventricular arrhythmia dose dependently .after I/R. As compared with I/R group, the agonist middle and high concentration(10-7mol/L and 2×10-7mol/L) could reduce the ventricular arrhythmia score and the incidence of ventricular arrhymia after reperfusion, in contrast , the inhibitor middle and high concentration（6×10-7mol/L and 1.2×10-6mol/L） could increase the ventricular arrhythmia score and the incidence of ventricular arrhymia after reperfusionimportant role in ischemia reperfusion ventricular arrhythmia of isolated rat hearts. Regulating ILK activity may be a potential therapeutic for reperfusion ventricular arrhythmias. Key words ILK; ischemia- reperfusion; ventricular arrhythmia; *基金项目：973项目（2013CB531104）；国家自然科学基金资助项目； 作者简介：周平，女，在读硕士研究生，联系方式：固话：023 E-mail：zhouping6545@163