子宫内膜癌I型和II型全基因表达谱初步分析-南方医科大学web2.doc

子宫内膜癌I型和II型全基因表达谱初步分析 作者：郭遂群1；邢福祺1；庞占军1；方唯意1；刘国炳1　　(南方医科大学1南方医院妇产科，2肿瘤研究所，广东??广州??510515) 摘要：目的??分析子宫内膜癌I型和II型全基因表达谱。方法??利用6张Affymetrix全基因组基因芯片，分析两者之间在表达谱上的差异，结合生物信息分析。 结果??许多基因明显在雌激素依赖型子宫内膜癌中高表达，其中，一些基因涉及了雌激素依赖型中雌激素的代谢与转化，另外一些基因可能为雌激素调节基因。CYP2C9参与了雌激素酮硫酸盐转化为16-羟基硫酸盐代谢；DDC能与类固醇受体结合，增强类固醇受体的转录活性。结论??某些基因在雌激素依赖型子宫内膜癌中高表达，为进一步研究此类基因在子宫内膜癌发病过程的作用及预后奠定了基础。 关键词：子宫内膜肿瘤；基因芯片；生物信息分析；雌激素依赖型 中图分类号：R737.33; R392.3??文献标识码：A?? 文章编号：1673-4254(2006)06-0734-04 Preliminary study of gene expression profiling in human type I and II endometrial carcinomaGUO Sui-qun1； XING Fu-qi1； PANG Zhan-jun1； FANG Wei-yi2； LIU Guo-bing11Department of Gynaecology and Obstetrics, Nanfang Hospital, 2Institute of Cancer Research, Southern Medical University, Guangzhou 510515, ChinaAbstract: Objective To study gene expression profiling in human type I and II endometrial carcinoma. Methods Six Affymetrix human genome genechips were utilized to investigate the differences in gene expression profiles between type I and II endometrial carcinoma with bioinformatic analysis. Results Many genes were highly expressed in estrogen-dependent endometrial carcinoma, and some of them were involved in the metabolism and conversion of estrogen, while some others in estrogen regulation. CYP2C9, for instance, was involved in the conversion of estrogen sulfate to 16-hydroxy sulfate metabolite, DDC in estrogen-dependent pathogenesis of endometrial carcinoma possibly by DDC interaction with AR to enhance steroid receptor transcription. Conclusion High expression of these genes in estrogen-dependent endometrial carcinoma may provide insights into their roles in the pathogenesis and prognosis of this malignancy.Key words: endometrial neoplasm; genechips; bioinformatic analysis; estrogen-dependent收稿日期：2006-01-18基金项目：广东省自然科学基金(5004777)Natural Science Foundation of Guangdong Province(5004777)作者简介：郭遂群(1964-)，男，在读博士研究生，副教授、副主任医师，电话：020E-mail:guosq@ ????在美国子宫内膜癌是一种最常见的女性生殖道恶性肿瘤，在所有的肿瘤中排名第四，仅次于乳腺癌、肺癌和大肠癌[1]。