生物工程专业英语作业课件.pptVIP

专业英语 目录 1.标题和摘要 2.主要内容 3.结论 4.专业词汇 5.经典句例 1.题名和摘要 题名：Characterization and immobilization of liposome-bound cellulase for hydrolysis of insoluble cellulose 翻译：能水解不溶性纤维素的脂质体纤维素酶的特征及固定化 Abstract： The liposome-bound cellulase was prepared by covalently coupling cellulase with the enzyme-free liposomes bearing aldehyde groups so that cellulase was located solely on the outer membrane of liposomes. The modified cellulase possessed the higher activity efficiency and lipid-based specific activity than the cellulase-containing liposomes reported previously. The enzyme-free liposomes bearing aldehyde groups were covalently immobilized with the chitosan gel beads and the free cellulase was coupled with the treated gel beads to prepare the immobilized liposome-bound cellulase. The activity efficiency of the immobilized liposome-bound cellulase was much higher than that of the conventionally immobilized cellulase. The results on reusability of the immobilized liposome-bound cellulase in the hydrolysis of either soluble or insoluble cellulose showed that the immobilized liposome-bound cellulase had the higher remaining cellulase activity and reusability than the conventionally immobilized cellulase for the hydrolysis of either type of cellulose. The liposomal membrane was suggested to be efficient in maintaining the cellulase activity during the hydrolysis. 翻译 摘要：脂质体纤维素酶是利用纤维素酶与脂质体上醛基通过共价键结合，使纤维素固定在脂质体的外膜上。修改后的纤维素酶活比以前报道的含脂质体纤维素酶活更高。含有醛基的脂质体耦合脱乙酰化壳多糖凝胶颗粒和游离纤维素酶形成了固定化脂质体纤维素酶。固定的脂质体纤维素酶的酶活远远高于传统固定的纤维素酶活。对固定化脂质体纤维素酶水解可溶性和不溶性的纤维素的重复利用度的实验结果表明固定化的脂质体纤维素酶水解纤维素比传统固定化纤维素酶有更高的活性和重复利用度。脂质膜在水解过程中高效的维持了纤维素酶的活性。 Keywords: Enzymatic cellulose hydrolysis;Liposome-bound cellulase;Immobilized cellulase; Chitosan get 关键词：纤维素酶水解;脂质体结合纤维素酶固定化纤维素酶;壳聚糖得到 2.主要内容 1.脂质体纤维素酶和固定化脂质体纤维素酶的制备 分析 图中显示，醛基首先引入到脂质体的表面上，这种含有醛基的脂质体能够同时与纤维素酶和聚乙酰化壳多糖凝胶颗粒结合。脂质体单独与纤维素酶耦合即为脂质体纤维素酶，若与两者同时结合则生成固定化脂质体纤维素酶。 2.脂质体纤维素酶水解可溶性纤维素的催化性能 分析 基于脂质特异活性可以用来横量纤维素酶的催