丹参酮IIA对非酒精性脂肪肝大鼠肝脏脂质过氧化的影响.docVIP

疏肝活络汤对非酒精性脂肪肝大鼠肝脏脂质过氧化的影响 章一凡 朱雄雄 任光荣 苏州市中医医院（江苏苏州 215003） 摘要 目的： 研究疏肝活络汤对非酒精性脂肪肝病(NAFLD)大鼠肝脏脂质过氧化的影响，并初步探讨其作用机制。方法:建立高脂饲料诱发大鼠NAFLD模型,8周后将模型成功大鼠随机分为模型组、疏肝活络汤组和阳性药易善复组，每组均给予高脂饲料及相应药物，正常对照组喂以基础饲料。连续给药6周后比较各组间肝脏指数、测定血脂、血清谷丙转移酶(ALT)、谷草转移酶(ASL)活性和超氧化物歧化酶(SOD)、丙二醛(MDA)的含量；检测肝脂质、肝脏组织中SOD、MDA含量并进行肝脏病理学检查。结果: 疏肝活络汤可显著降低NAFLD模型大鼠肝脏指数、降低血清和肝组织中TG、TC、MDA水平，降低血清LDL-C含量和ALT、AST活性；增加血清和肝组织中SOD活性以及HDL-C含量,与模型组相比差异显著，并可明显减少肝细胞浆内脂滴分布的程度和炎症范围。结论:疏肝活络汤可通过改善肝功能及脂质代谢紊乱，减少肝中脂质沉积，减轻肝组织损伤，对高脂饮食诱发的非酒精性脂肪肝有一定治疗作用。 关键词：疏肝活络汤；非酒精性脂肪肝病；大鼠模型；脂质代谢 Experimental Study on Effects of Shuganhuoluo Decoction on Nonalcoholic Fatty Liver Lipid Peroxidation Rats Zhang Yifan, Zhu Xiongxiong, Ren Guangrong Suzhou Municipal Hospital of Traditional Chinese Medicine ( Suzhou, 215003) Abstract：Objective: To study the effects of Shuganhuoluo Decoction (SD) on nonalcoholic fatty liver disease (NAFLD) and its possible mechanism. Methods： NAFLD model rats were induced by high fat diet. Polyene Phosphatidylcholine Capsules (PPC) was closen to be the control medicine. The pathological changes of liver, levels of blood-fat, ALT, AST, SOD, MDA in serum, levels of TC, TG, SOD, MDA in liver were monitored. Results: SD could decrease The level of TC，LDL-C，ALT , AST, MDA inlasma and hepatic index，the level of TGTC in liver，: 章一凡(1966-)，男，江苏人研究方向while increase the level of SOD in serum and liver and increase the level of HDL-C content, and also reduce distribution of lipid droplet on degree and scope in liver cell. Conclusion: SD could treat the nonalcoholic fatty liver of rats induced by feeding with high fat diet through improving fat metabolism disorder，redulevels of liver lipid, lipid peroxide products and the fatty deposited in liver，and imroving the function of periphery adipose tissue. Keywords: Shuganhuoluo Decoction (SD); nonalcoholic fatty liver disease; the model of rats; lipid peroxidation metabolism 随着我国老龄化人口增加，人们生活方式转变及饮食结构西化，我国非酒精性脂肪肝(nonalcoholic fatty liver diseases，NAFLD)发病率逐年增长。NAFLD是一类与酒精性肝病相似但无