Noth2调控舌鳞癌干细胞的增殖侵袭和迁移.doc

Noth2调控舌鳞癌干细胞的增殖、侵袭和迁移 葛臻琼1，闫恩利2，孙学蓉3，任雪梅2，肖 海4(六盘水市人民医院，1口腔科，3肿瘤科，贵州省六盘水市 553000；2郑州大学第二附属医院康复科，河南省郑州市 450000；4贵州医科大学天然药物资源优效利用重点实验室，贵州省贵阳市 550000) 引用本文：葛臻琼，闫恩利，孙学蓉，任雪梅，肖海. Noth2调控舌鳞癌干细胞的增殖、侵袭和迁移[J].中国组织工程研究，2016，20(45):6753-6759. DOI: 10.3969/j.issn.2095-4344.2016.45.010 ORCID: 0000-0003-2450-7271(葛臻琼) 文章快速阅读： 文题释义： Notch2：Notch蛋白家族不但参与细胞的增殖、分化、凋亡及器官发育，且在肿瘤的发生和发展中发挥着重要的作用。Notch2已被报道与多种肿瘤的发生与发展相关，但是其作用较为复杂。有研究报道其作为癌基因，促进癌症的发生和发展。然而也有研究报道其在一些肿瘤中是低表达的，对癌症起抑制作用。 肿瘤干细胞：是肿瘤细胞内存在的一小部分具有干细胞特性的细胞，拥有无限增殖、自我更新和多系分化的能力。这些细胞可能能够驱动肿瘤的生长、传播和转移。治疗后残余的“肿瘤干细胞”因为放疗抗性及化疗耐药性等而难以被杀死，最终可能导致肿瘤复发。 Notch2在舌鳞癌干细胞ALDHbr)的表达量Notch2 regulates proliferation, invasion and migration of tongue squamous cell carcinoma stem cells Ge Zhen-qiong1, Yan En-li2, Sun Xue-rong3, Ren Xue-mei2, Xiao Hai4 (1Department of Stomatology, 3Department of Oncology, People’s Hospital of Liu Pan Shui, Liupanshui 553000, Guizhou Province, China; 2Department of Rehabilitation, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China; 4Key Laboratory of Natural Resources and Utilization of Natural Resources of Guizhou Medical University, Guiyang 550000, Guizhou Province, China) Abstract BACKGROUND: Previous studies have found that the expression of Notch2 is up-regulated in tongue squamous cell carcinoma (TSCC) stem cells, but the effect of Notch2 on TSCC remains unclear. OBJECTIVE: To explore the effect of Notch2 on the proliferation, invasion and migration of TSCC stem cells (ALDHbr). METHODS: ALDHbr cells were enriched and separated by fluorescence-activated cell sorting technique from Tca8113 cells cultured in using serum-free medium, and Notch2 expression in ordinary TSCC cells (ALDHlow) and ALDHbr cells was detected by western blot. The ALDHbr cells transfected with Notch2 shRNA (sh-Notch2) by liposome served as experimental group, and ALDHbr cells transfected with sh-control were as control group. The effects of Notch2 on the proliferat