Mycology Austin Community College真菌学奥斯汀社区学院.pptVIP

Laboratory Methods in Medical Mycology Stains Used in Mycology Fluorescent Antibody Stain - simple, sensitive, and specific. Applications for many different fungal organisms Papanicolaou Stain - good for initial differentiation of dimorphic fungi. Works well on sputum smears Gram Stain - most fungi are gram positive; Actinomyces and Nocardia are gram variable Modified Acid-Fast Stain - differentiates acid-fast Nocardia from other aerobic Actinomyces Giemsa Stain – use on blood and bone marrow specimens. India Ink - demonstrates the capsule of Cryptococcus neoformans in CSF specimens Laboratory Methods in Medical Mycology Fungal Culturing Tubed media is used rather than plated media because: there is less chance for spore release into the environment less chance for dehydration ease of storage The agar in a tube is inoculated in a straight line. The agar on plates is Inoculated like a large S so that rapid growing fungi can removed Incubation should be aerobic (and anaerobic if Actinomycetes are suspected) Incubate cultures at room temperature also at 37C if dimorphic fungi are suspected Cultures are kept for 4 weeks should be examined every other day Systemic pathogens often require 10 days to 2 weeks Laboratory Methods in Medical Mycology Media Used for Isolation of Fungi Sabourauds dextrose agar (Sab-Dex) - classic medium, recommended for most studies Sabourauds dextrose agar with chloramphenicol - chloramphenicol inhibits bacterial growth Mycosel agar - commercially available. Contains chloramphenicol to inhibit bacterial growth; cycloheximide to inhibit saprophytic fungi and some yeasts (including C. neoformans) Brain heart infusion slant (BHI) - more enriched than Sab-Dex. Used to recover H. capsulatum Potato-dextrose agar (PDA) and Corn-meal agar - used in slide cultures; these induce spore formation, which greatly aids in identification Laboratory Methods in Medical Mycology Special Applications Media Caffeic Acid Agar - Cryptococcus neoformans will