MedicalParasitologyLab.ppt

Hymenolepis nana scolex Mature proglottid Gravid proglottid The scolex is the head portion of the worm, and is equipped with a slit-like groove (the bothrium) for attachment to the intestine. Un embryonated egg passed in feces and become embryonated in water * * * LAB 5 Flotation The flotation procedure permits the separation of protozoan cysts and certain eggs from excess debris through the use of a liquid with a high specific gravity. The parasitic elements are recovered in the surface film, and the debris remains in the bottom of the tube. This technique yields a cleaner preparation than the sedimentation procedure. Zinc Sulphate technique Why can’t all helminth eggs be recovered using the flotation concentration rather than the sedimentation concentration? Some helminth eggs are quite heavy (unfertilized Ascaris eggs) and will not float, even using zinc sulfate with a specific gravity of 1.20. Other helminth eggs are operculated; when the egg is placed in a high specific gravity solution, the operculum “pops” open and the egg fills with fluid and sinks to the bottom of the tube. Thus, both the surface film and the sediment should be examined before reporting the specimen as negative. Materials and Method Zinc sulphate solution with specific gravity 1.18. Mix 330gm dry zinc sulphate in 670ml distilled water. Use the hydrometer or densitometer to adjust specific gravity around 1.18 Notes : Warning! May cause skin irritation. May cause eye irritation and possible burns. Evaporation Rate: ether Chemical Stability: Stable under normal temperatures and pressures.   Zinc Sulphate centrifugal flotation technique Transfer about 0.5 teaspoon of stool to a test tube containing 1-2 ml of water and comminute thoroughly then fill the tube to within 2-3 mm of the top with water. Centrifuge at 1500 rpm for 1 mint. Discard supernatant fluid. If the sample contains a large amount of material that floats in water, you may want to wash the sample before doing the fl