FTICRMS原理及应用郭寅龙中国科学院上海有机化学研究所上海.doc

FTICRMS原理及应用 郭寅龙 中国科学院上海有机化学研究所 上海质谱中心 第一部分：FTICRMS原理 Fourier Transform Ion Cyclotron Resonance Mass Spectrometry 傅立叶变换离子回旋共振质谱 一、仪器结构 Schematic of External Ion Source FTMS with ESI Source Cylindrical Ion Cyclotron Resonance Cell  (ICR Cell, ICR Trap) Basic Features of FTMS Instrumentation 二、基本原理 Basic Principles: Ion Cyclotron Motion Principles of Fourier Transform Mass Spectrometry Cyclotron Motion For a stable circular orbit the centrifugal force outward exactly balances the magnetic force inward. Theory : Detection Excitation and Detection Principles of Fourier Transform Mass Spectrometry Fourier Signal Analysis FTMS Event Sequence FTMS Ion Excitation Excitation of the Ion Cyclotron Motion 三、离子的产生 1.激光解吸离子化（LD） 1) 激光解吸离子化 2)基质辅助激光解吸离子化(MALDI） 2.电喷雾离子化（ESI） 四、离子的传输和捕集 Voltage on Trapping Plate 1 is lowered as the ions approach the analyzer cell 五、提高分辨率的方法 1。提高离子观测时间（增加采样点数） Effect of Transient Length on Resolution Digitizing the FTMS signal for 1.049 seconds instead of 0.262 seconds gives x4 higher mass resolution Effect of Transient Length on Resolution Fourier Signal Analysis Window Functions Acquiring a time-domain signal for a finite time is equivalent to multiplying the actual signal by a rectangular window function. Only the data in this 搕ime-window?is stored in the computer and subjected to an FFT.
Window Functions: Abrupt truncation of the time-domain signal causes auxiliary wiggles on both sides of the main peak. These artifacts are called Gibbs oscillations and are removed by multiplying the signal by an apodization function. Popular apodization functions are the Hanning Window function and the Blackman-Harris Window function. Apodization Functions Change the Peak Shape 2。Zero Filling Improves the Peak Shape Two zero fills are used normally to increase the number of data points across a peak After taking 2M of data, another 6M of zeros is appended to the transient prior to calculating the FFT 3.提高分析池真空 4.减小扫描的质量范围 MALD