8.Conclusion-中国医学科学院基础医学研究所北京协和医学院.doc

心输出量研究实验 陈典，弓孟春，花苏榕，景灏，申曦，杨明 中国协和医科大学，临床医学系 2007/03 摘要 实验目的： 实验方法：以蟾蜍心脏为实验材料，通过改变灌注液面高度改变前负荷（同时也就改变了回心血量）；通过改变动脉插管的液面高度改变后负荷；通过加入肾上腺素模拟药物对心脏的影响；通过改变灌注液温度模拟温度对心肌工作能力的影响；通过电刺激抢先占领窦房结的起搏功能，从而人为改变心率。以上方法为改变心脏的泵条件，从而改变心脏的工作情况，供观察和记录。 实验结果： 实验结论： 关键词 蛙心、心输出量、心力储备、搏出量储备、收缩期储备、心率储备、前负荷、后负荷] Abstract Object Analyze the influencing factors of the function of the pumping heart. Research on the two aspects of the cardiac reserve棗reserve of the stroke volume and heart rate棗through the methods of changing the perfusion condition, such as change the preload, afterload, the temperature, heart rate or add some kind of drugs. Then observe and take down the minute volume in order to do succeeding analysis. We can also observe the influence of several factors on the heart rate. Methods Take the heart of the toad as the experimental material. Change the preload by changing the height of the perfuse liquid, Renne’s solution( at the same time, we change the liquid volume coming back to the heart); Change the afterload by changing the height of the perfuse liquid also, but in the glass branching tube inserted into the main artery; Add epinephrine to mimic the condition that drugs are used; Change the temperature to observe its influence on the working heart; To seize the pacing function of the venous sinus and shift it to be under our control, we put the electric stimulation on the heart. So we can adjust the heart rate as we would like to; Through the methods above, we can observe the work situation of the heart and do analysis. Results The result of the experiment is a complicated one. When the preload is changed, in a certain range, the minute volume goes up according to the growing preload. But if we go beyond the range, the volume goes down; Similar results occur if we change the afterload , the temperature, the heart rate. Just the value of the range and the percentage of the separate phases of the curve are not the same. By changing the heart rate we get a strange curve with t