染料壳聚糖微球对人血清蛋白吸附性能的研究.pdf

染料壳聚糖微球对人血清蛋白吸附性能的研究 1 2 3 王璘 ，张静 ，张政朴 （1.南开大学化学学院，天津 300071，中国；2. 南开大学化学学院，天津 300071，中国； 3. 南开大学化学学院，天津 300071，中国） 摘要：本文以Cibacron Blue F3GA染料为配体，1，6-己二胺为间隔臂，交联壳聚糖微球为基质，制备 了一种染料亲和色谱的固定相，对血清白蛋白进行吸附分离，从而为通过色谱实验来对血清白蛋白进行 分离研究提供了初步的实验依据。 文中以壳聚糖为原料，利用反相悬浮交联法，以戊二醛为交联剂，制备了不同交联度的壳聚糖微球， 并对该微球进行羟丙基氯化和胺基化，并偶联色素配体Cibacron Blue F3GA，得到一种新型染料亲和吸 附剂。以人血清白蛋白（HSA）为目标蛋白，考察了该染料亲和吸附剂在不同PH值、离子强度及不同HSA 浓度下对 HSA 的吸附性能。结果发现：HSA 最大平衡吸附量为 79.5 mg/g，出现在 pH=5.0，也就是 HSA 的等电点；随着 HSA 浓度增大，蛋白的吸附量也增大，吸附行为满足 Langmuir 吸附等温式；随着离子 强度的增加，HSA的吸附量明显减小。 关键词：交联壳聚糖微球 Cibacron Blue F3GA HSA 吸附 洗脱 Adsorption of Human Serum Albumin to Cibacron Blue F3GA-derived Chitosan Microspheres WANG Lin 1, ZHANG Jing 2, ZHANG Zheng-pu3 (1.College of Chemistry, Nankai University, Tianjin 300071, China; 2. College of Chemistry, Nankai University, Tianjin 300071, China; 3. College of Chemistry, Nankai University, Tianjin 300071, China) Abstract ： In this paper, a series of novel aminated chitosan microbeads were prepared from chitosan through inverse suspension crosslinking, hydroxylpropyl - chlorination and amination. Affinity dye-ligand, Cibacron Blue F3GA, was covalently coupled with aminated chitosan microspheres via nucleophilic reaction. Albumin adsorption onto Cibacron Blue F3GA-derived chitosan microspheres was investigated. The effects of medium pH, ionic strength and initial BSA concentration on the adsorption capacity of CBF-CS microspheres were studied The HSA equilibrium adsorption capacity was 79.5 mg/g polymer, which was observed at pH = 5.0, the isoelectric point of HSA. The adsorption capacity increases with the increasing of BSA concentra