Theoperon,清华,基因.pptVIP

Chapter 10 The operon 10.1 Introduction 10.2 Regulation can be negative or positive 10.3 Structural gene clusters are coordinately controlled 10.4 The lac genes are controlled by a repressor 10.5 The lac operon can be induced 10.6 Repressor is controlled by a small molecule inducer 10.7 cis-acting constitutive mutations identify the operator 10.8 trans-acting mutations identify the regulator gene 10.9 Multimeric proteins have special genetic properties 10.10 Repressor protein binds to the operator 10.11 Binding of inducer releases repressor from the operator 10.12 Repressor is a tetramer 10.13 Repressor binds to three operators and interacts with RNA polymerase10.14 Repressor is always bound to DNA 10.15 The operator competes with low-affinity sites to bind repressor 10.16 Repression can occur at multiple loci 10.17 Distinguishing positive and negative control 10.18 Catabolite repression involves the inducer cyclic AMP and the activator CAP10.19 CAP functions in different ways in different target operons 10.20 CAP bends DNA 10.21 The stringent response produces (p)ppGpp 10.22 (p)ppGpp is produced by the ribosome 10.23 pGpp has many effects 10.24 Translation can be regulated 10.25 r-protein synthesis is controlled by autogeneous regulation 10.26 Phage T4 p32 is controlled by an autogenous circuit 10.27 Autogenous regulation is often used to control synthesis of macromolecular assemblies 10.28 Alternative secondary structures control attenuation 10.29 The tryptophan operon is controlled by attenuation 10.30 Attenuation can be controlled by translation 10.31 Small RNA molecules can regulate translation 10.32 Antisense RNA can be used to inactivate gene expression Operator is the site on DNA at which a repressor protein binds to prevent transcription from initiating at the adjacent promoter.Repressor protein binds to operator on DNA or RNA to prevent transcription or translation, respectively.Structural gene codes for any RNA or prote