RP-HPLC法测定抗妇炎泡腾栓中蛇床子素和盐酸小檗碱的.PDFVIP

中国药房 2008年第 19卷第 15期 Ch ina Pharmacy 2008 V ol. 19 No. 15 # 1157# RP- HPLC 法测定抗妇炎泡腾栓中蛇床子素和盐酸小檗碱的含量 $ 张亚洲 1 ? , 骆莉莉 1 , 董立莎 1 , 蔡少青 2 # ( 11贵阳中医学院, 贵阳市 550002; 21北京大学药学院, 北京市 100083) 中图分类号 R2831623; R92712 文献标识码 A 文章编号 1001- 0408( 2008) 15- 1157- 03 摘 要 目的:建立以反相高效液相色谱法测定抗妇炎泡腾栓中蛇床子素和盐酸小檗碱含量的方法。方法:色谱柱为 Diamonsil C 18 ( 250 mm @ 416 mm , 5 Lm ) , 流动相为乙腈- 012%磷酸溶液( 52B 48, 每 100 mL 含 011 g 十二烷基磺酸钠) ,检测波长为 322 nm, 流速为 1 mL #min - 1,柱温为 30 e 。结果:蛇床子素、盐酸小檗碱进样量分别在 01019 8~ 01396 0 Lg ( r= 01999 97)、01056 65~ 11133 00 Lg ( r = 01999 99) 范围内与各自峰面积积分值呈良好线性关系; 平均加样回收率分别为 101106% ( R SD = 1110% )、 101188% ( R SD = 0198% )。结论: 本方法简便、准确、重现性好,可用于抗妇炎泡腾栓的质量控制。 关键词 抗妇炎泡腾栓;反相高效液相色谱法;蛇床子素;盐酸小檗碱;含量测定 Determination of Osthol and Berberine in Kangfuyan Effervescent Suppository by RP- HPLC ZHANG Ya- zhou, LUO Li- li, DONG Li- sha( Guiy ang College of T radit ional Chinese M edicine, Guiyang 550002, China) CA I Shao- qing( School of Pharmaceut ical Sciences, Beijing U niv ersity, Beijing 100083, China) ABSTRACT OBJECT IVE: To establishes an HPLC method fo r determination o f the content s of Ost hol and Berber ine in Kang fuyan effervescent suppository1 METHODS: HPLC w as car r ied out on a colum n of Diamonsil ( 250 mm @ 416 mm , 5 Lm) w ith mobile phase consisted o f acetonit rile- 012% phospha te acid ( 52 B48, Laury l sulfonic acid sodium 011 g w as cont ained in every 100 mL) w it h flow rate at 1 mL #m in - 11T he det ection w aveleng th w as set at 322 nm and the co lum n tem perat ure was set at room temperature1 RESU LTS: T he linear range w as 01019 8~ 01396 0 Lg ( r = 01999 97) f or Ost hole versus 01056 65~ 11133 00 Lg ( r = 01999 99) f or Berberine1 The average recover ies o f Ostho l and Berber ine w ere 101106% ( RSD= 1110%) and 101188% ( R SD = 0198% ) , respect ively1 CONCLU SION: T his m ethod is simple, accurate and reproducible, and applicable for t he qualit y contro l o f Kang fuyan e ffervescent supposit ory1 KEY WORDS Kang fuyan ef fervescent supposit ory; RP- HPLC; Os