毕业论文--丁二酰化壳寡糖稀土配合物的合成表征及与DNA的作用.docVIP

丁二酰化壳寡糖稀土配合物的合成表征及与DNA的作用 摘要：以丁二酸酐和壳寡糖为原料在在室温下合成了水溶性的丁二酰化壳寡糖和稀土离子La3+、Nd3+、Eu3+ 的配合物。利用红外光谱、紫外吸收光谱和差热重热重分析对配合物进行了表征，并且以中兴红（NR）作探针研究了壳寡糖稀土配合物与鲱鱼精DNA的相互作用,获得了结合比。实验结果显示：结合比为nCS－La:nDNA=?nBCS－Eu:nDNA=?nBCS－Nd:nDNA=?随着DNA的加入，配合物在275nm处的紫外吸收峰不断增强，同时在528nm左右的荧光发射随着DNA浓度的增大均逐渐减小，说明配合物和DNA之间的作用方式为静电作用方式，NR与DNA之间的作用方式是嵌插作用方式，两者均影响其调控与表达，进而抑制肿瘤细胞的生长。 关键词：丁二酸酐；壳寡糖； 稀土配合物；鲱鱼精DNA；作用方式 Synthesis , characterization and Interaction with Herring Sperm DNA of Succinic acylation Chitosan Oligosaccharide-rare earth complexs Wang Xia Li xiao Fang ( School of Life Sciences and Chemistry ,Tianshui Normal University ,The class 08 grade 1 ,Tianshui 741000,Gansu, China ) as a probe.the combining ratio of the binary complexs were obtained. The results indicate that the combining ratio of the binary complex is nCS－La:nDNA = ?nBCS－Eu:nDNA =?nBCS－Nd:nDNA = ? Along with DNA to join , the ultraviolet absorption peaks of complexs are constantly strengthen in 275nm, while the fluorescence-emission Along with the increase of concentration of DNA are gradually decrease in 528nm .Study indicate the chief action model between complex and DNA is electrostatic effect way, and the action model between NR and DNA is an intercalation binding . These interaction mode affects the distribution and expression of gene and resists the groeth of cancer cells. Key words Acylation succinic anhydride ； Chitooligosal-charide ； rare earth complexs； Herring Sperm DNA ；action mode 图1 CS和BCS的红外光谱图 Fig .1 FT-IR spectra of CS and BCS 图2 BCS、BCS- Eu、BCS- La和BCS -Nd的红外光谱图 Fig .2 FT-IR spectra of BCS、BCS- Eu、BCS- La and BCS -Nd BCS以及其配合物的红外光谱如图2所示。BCS－Eu、BCS－La、BCS－Nd的主要成分均为BCS, 所以4种谱图基本相似。 1364cm-1左右的吸收峰是－CH3的对称变形振动峰，775.70cm-1左右的吸收峰是－CH2的平面摇摆振动峰，但在某些波数处吸收峰又表现出明显的不同，BCS在3364.80cm-1处的宽且强的吸收峰在BCS－Eu、BCS－La、BCS－Nd中该吸收峰向高波数移动且逐渐变弱变窄，1618.96cm-1处的吸收峰向低波数移动，BCS在1776.64cm-1处的吸收峰在其配合物的谱图中消失，107