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Basic helix-loop-helix transcription factor from wild rice improves tolerance to salt osmotic.pdf

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Basic helix-loop-helix transcription factor from wild rice improves tolerance to salt osmotic

ARTICLE IN PRESSJournal of Plant Physiology 166 (2009) 12961617/$ - s doi:10.1016/j. Abbreviation CRT/DRE, C-re protein; GUS, b transcriptional Correspond E-mail addr 1Contributedwww.elsevier.de/jplphBasic helix-loop-helix transcription factor from wild rice (OrbHLH2) improves tolerance to salt- and osmotic stress in Arabidopsis Jing Zhoua,b,1, Fei Lia,b,1, Jin-lan Wanga,b, Yun Maa, Kang Chonga, Yun-yuan Xua,aKey Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing 100093, China bGraduate School of the Chinese Academy of Sciences, Beijing 100049, China Received 22 September 2008; received in revised form 5 February 2009; accepted 5 February 2009KEYWORDS bHLH; ICE1; OrbHLH2; Salt tolerance; Transcription factoree front matter 2009 jplph.2009.02.007 s: ABA, abscisic acid; peat/dehydration respo -glucuronidase; ICE1, In polymerase chain react ing author. Tel.: +86 10 ess: xuyy@ ( equally to this work.Summary Salt stress adversely affects plant growth and development. Some plants reduce the damage of high-salt stress by expressing a series of salt-responsive genes. Studies of the molecular mechanism of the salt-stress response have focused on the characterization of components involved in signal perception and transduction. In the present work, we cloned and characterized a basic helix-loop-helix (bHLH) encoding gene, OrbHLH2, from wild rice (Oryza rufipogon), which encodes a homologue protein of ICE1 in Arabidopsis. OrbHLH2 protein localized in the nucleus. Overexpression of OrbHLH2 in Arabidopsis conferred increased tolerance to salt and osmotic stress, and the stress-responsive genes DREB1A/CBF3, RD29A, COR15A and KIN1 were upregulated in transgenic plants. Abscisic acid (ABA) treatment showed a similar effect on the seed germination or transcriptional expression of stress- responsive genes in both wild type and OrbHLH2-overexpressed plants, which impli