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Electrochemical studies of DNA immobilization onto the2
Available online at /locate/yabio
ANALYTICAL
BIOCHEMISTRY
Analytical Biochemistry 375 (2008) 331–338Electrochemical studies of DNA immobilization onto the
azide-terminated monolayers and its interaction with taxol
Ali Mehdinia a, S. Habib Kazemi a, S. Zahra Bathaie b, Abdolhamid Alizadeh c,
Mojtaba Shamsipur c, Mir Fazlollah Mousavi a,*
aDepartment of Chemistry, Tarbiat Modares University, Tehran, Iran
bDepartment of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
cDepartment of Chemistry, Razi University, Kermanshah, Iran
Received 28 November 2007
Available online 10 January 2008Abstract
A surface modification procedure for the creation of self-assembled monolayers (SAMs) that can be used as a scaffold for double-
stranded DNA (dsDNA) incorporation onto the gold surfaces is described. The SAMs of an azidohexane thiol derivative were prepared
on the Au electrode and then used for the immobilization of dsDNA. The electrochemical characteristics of dsDNA onto the SAM-mod-
ified gold electrode were investigated by cyclic voltammetry and electrochemical impedance spectroscopy, and the surface concentration
of dsDNA onto the SAMs surface was estimated. The interaction of dsDNA with the anticancer drug, taxol (paclitaxel), was also studied
on the surface of DNA/SAM/Au electrode. The observed decrease in the guanine oxidation peak current was used to monitor the inter-
action of taxol with DNA. The resulting Langmuir isotherm for taxol binding to DNA at the modified electrode was used to evaluate the
binding constant of taxol–DNA. The results obtained supported the groove binding interaction of taxol with DNA. The modified elec-
trode was used as a sensitive sensor for quantification of taxol in human serum sample.
2008 Elsevier Inc. All rights reserved.
Keywords: Self-assembled monolayers; DNA; Taxol; Cyclic voltammetry; Electrochemical impedance spectroscopyA major challenge in the immobilization of a variety of
biomolecules
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