原创力文档- 1、本文档共6页，可阅读全部内容。
- 2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
- 3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
查看更多
real-time PCR vs automated ELISA for Salmonella
Evaluation of real-time PCR vs automated ELISA and
a conventional culture method using a semi-solid medium
for detection of Salmonella
M. Uyttendaele, K. Vanwildemeersch and J. Debevere
Laboratory of Food Microbiology and Food Preservation, Department of Food Science and Nutrition, Faculty of Agricultural and Applied
Biological Sciences, University of Gent, Belgium
2003/0408: received 16 May 2003, revised 24 July 2003 and accepted 26 July 2003
ABSTRACT
M. UYTTENDAELE, K . VANWILDEMEERSCH AND J. DEBEVERE. 2003.
Aims: Evaluation of iQ-Check PCR Salmonella for Salmonella detection in artificially and naturally contaminated
food and environmental field samples.
Methods and Results: Artificially contaminated samples (poultry meat and ground red meat) subjected to
cold- and freeze-stress, and 120 naturally contaminated samples (swabs and meat) were tested for Salmonella using
the diagnostic semi-solid Salmonella medium (DIASALM) method, the Vidas assay and the iQ-Check PCR assay
after 24 h enrichment in buffered peptone water.2
Conclusions: Both the iQ-Check PCR and the Vidas assay provide a rapid and user friendly screening method for
detection of Salmonella. False negative samples were obtained for the inoculated samples using both the iQ-Check
PCR assay and the Vidas method when Salmonella cells were severely stressed. In total 45 of 120 naturally
contaminated field samples showed Salmonella positive using the DIASALM method. The agreement percentage
with the DIASALM method was respectively 92% for the iQ-Check PCR and 95% for the Vidas method.
Significance and Impact of the Study: False-negative samples were obtained for the inoculated samples
using both the iQ-Check PCR assay and the Vidas method when Salmonella cells were severely stressed, e.g.
freezing at )18C for 7 days. Of the 120 naturally contaminated field samples 45 showed Salmonella positive
using the DIASALM method. The agreement percentage with the DIASALM method was 92% for the iQ-Check
PCR and 95% for
您可能关注的文档
- on Pancultural self-enhancement.pdf
- On the Casimir effect for parallel plates in the spacetime with one extra compactified dime.pdf
- On the distribution of points in projective space of bounded height.pdf
- On the discrepancy principle for the dynamical systems method.pdf
- On The Field Theory Limit Of D-Instantons.pdf
- On The Effects of Path Correlation in Multi-Path Video Communications using FEC over Lossy.pdf
- On the Geometric Properties of AdS Instantons.pdf
- On the Hypermultiplet Moduli Space of Heterotic Compactifications with Small Instantons.pdf
- On the Double Scaling Limit of O(N) Vector Models in d=2.pdf
- ON THE INTRINSIC CHARM COMPONENT OF THE NUCLEON.pdf
文档评论（0）