Discovery of porcine microRNAs and profiling from skeletal muscle tissues during development.pdf

Discovery of Porcine microRNAs and Profiling from Skeletal Muscle Tissues during Development Ting-Hua Huang, Meng-Jin Zhu, Xin-Yun Li, Shu-Hong Zhao* Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan, People’s Republic of China Abstract MiRNAs (microRNAs) play critical roles in many important biological processes such as growth and development in mammals. In this study, we identified hundreds of porcine miRNA candidates through in silico prediction and analyzed their expression in developing skeletal muscle using microarray. Microarray screening using RNA samples prepared from a 33-day whole embryo and an extra embryo membrane validated 296 of the predicted candidates. Comparative expression profiling across samples of longissimus muscle collected from 33-day and 65-day post-gestation fetuses, as well as adult pigs, identified 140 differentially expressed miRNAs amongst the age groups investigated. The differentially expressed miRNAs showed seven distinctive types of expression patterns, suggesting possible involvement in certain biological processes. Five of the differentially expressed miRNAs were validated using real-time PCR. In silico analysis of the miRNA-mRNA interaction sites suggested that the potential mRNA targets of the differentially expressed miRNAs may play important roles in muscle growth and development. Citation: Huang T-H, Zhu M-J, Li X-Y, Zhao S-H (2008) Discovery of Porcine microRNAs and Profiling from Skeletal Muscle Tissues during Development. PLoS ONE 3(9): e3225. doi:10.1371/journal.pone.0003225 Editor: Suzannah Rutherford, Fred Hutchinson Cancer Research Center, United States of America Received March 24, 2008; Accepted August 15, 2008; Published September 16, 2008 Copyright:
2008 Huang et al. This is an open-access article distributed under the terms of the Creative Commons A