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Liquid–liquid extraction of lysozyme using a dye-modified ionic liquid.pdf

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Liquid–liquid extraction of lysozyme using a dye-modified ionic liquid

Aw [ w w t a ? K 1 m fl a b v m o o i h p t ( m I t p 0 dAvailable online at Journal of Chromatography A, 1193 (2008) 1–6 Liquid–liquid extraction of lysozyme using a dye-modified ionic liquid Yu-Ping Tzeng, Ching-Wei Shen, Tiing Yu ? Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30050, Taiwan Received 27 November 2007; received in revised form 1 February 2008; accepted 6 February 2008 Available online 11 April 2008 bstract An affinity-dye, Cibacron Blue 3GA (CB), derivatized organic salt [BMIM]3[CB] was synthesized for lysozyme extraction. This compound as formed by mixing an ionic liquid (IL) [BMIM][Cl] and the silver salt of CB. Liquid–liquid extractions of lysozyme from the aqueous and BMIM]3[CB] in [BMIM][PF6] solutions were examined in this study. The transfer of lysozyme from the aqueous phase to the IL phase decreased hile the pH of the aqueous phase increased. An extraction higher than 90% was observed at pH 4. Under a high ionic strength, the lysozyme ould transform back from the IL phase into the aqueous phase. Lysozyme molecules were almost quantitatively recovered from the IL phase to he aqueous solutions of 1 M KCl under pH 9–11. It appeared that the extraction was specific for lysozyme in contrast to cytochrome c, ovalbumin, nd bovine serum albumin. The extraction efficiency of the IL phase remained essentially the same after eight cycles of extraction. 3GA b T s m m a c I t t 3 b [ [ c i2008 Elsevier B.V. All rights reserved. eywords: Ionic liquid; Affinity extraction; Protein purification; Cibacron Blue . Introduction Ionic liquids (ILs) are molten organic salts which exhibit any attractive properties such as negligible volatility, non- ammability, thermal stability, and selective solubility. They re used in liquid–liquid extractions and are considered to e environmentally benign solvents because of their very low apor pressures. The designable solubilities for many organic olecules can sometimes be achieved by altering t