银杏总黄酮对肾缺血再灌注大鼠p-JNK表达的影响(朱家军等).docVIP

银杏总黄酮对肾缺血再灌注大鼠p-JNK表达的影响 朱家军1，夏安周2，王妍妍2，张探2, 李娜2 (1.灌云县人民医院，江苏灌云222200.徐州医学院，江苏徐州221004；) [摘要] 目的： 观察肾缺血再灌注损伤（IRI）后JNK活化的变化，并探讨银杏总黄酮（TFG）对其影响。方法： SD大鼠随机分为假手术组（Sham，n = 12)、缺血再灌注组（IR，n = 30）和银杏总黄酮处理组（TFG，n = 12）。采用夹闭双侧肾动静脉45 min然后松开动脉夹制备肾IRI模型。蛋白免疫印迹检测肾组织中p-JNK的表达，并用细胞凋亡原位末端标记（TUNEL法）检测肾小管上皮细胞凋亡。结果： IR组肾组织中p-JNK的表达在再灌注10 min 开始升高，再灌注30 min 达到高峰，再灌注1h有所降低，再灌注24h再次升高，同时该时段有大量肾小管上皮细胞凋亡；给予TFG处理，再灌注30min p-JNK表达明显减弱，再灌注24h细胞凋亡明显减少。结论：TFG 防治肾IRI机制可能与抑制??组织中JNK磷酸化的程度，减少肾小管上皮细胞凋亡有关。 [关键词] 缺血再灌注；银杏总黄酮；c-Jun 氨基末端激酶；肾 Effect of total flavone of Gingko on phosphorylation of JNK in rats with renal ischemia-reperfusion injury XIAAn-zhou1， ZHUJia-jun2 *， WANGYan-yan1，ZHANGTan1 ,LINa1 (1 Department of pharmacology , Xuzhou Medical college , Xuzhou 221002；2 Department of Anesthesiology, the People’s Hospital of Guanyunxian , Jiangsu, Lianyungang 222004) Abstract：Objective To investigate the change of phosphorylation of JNK in renal ischemia reperfusion injury（IRI）and study the effect of total flavone of Gingko（TFG）on JNK activation. Methods Male Sprague-Dawley rats were randomly divided into sham-operation group（Sham, n =12)，ischemia reperfusion groups（IR, n=30) and TFG groups（TFG, n=12). The models?were?established through clamping bilateral renal arteries for 45 min?and then reperfusion. Tubular cell apoptosis was confirmed by terminal deoxynucleotidyl transferase (TDT)-mediated dUTP-biotin nick end labeling (TUNEL) assay；The phosphorylation of JNK were detected by Western blotting analysis. Results In the IR group, the levels of tubular cell apoptosis were significantly increased; activation of JNK was observed in the kidney as early as 10 min after reperfusion，reaching its peak at 30 min and declined at 1 h，but increasd at 24 h after reperfusion. pretreatment with TFG decreased significantly the levels of tubular cell apoptosis; it also decreased the phosphorylation level of JNK compared with that in the IR group. Conclusion The results showed that the mechanism of TFG to pro