Huangetal.JZhejiangUniv.PDFVIP

Huang et al. / J Zhejiang Univ SCIENCE B 2006 7(5):373-376 373 Journal of Zhejiang University SCIENCE B ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) /jzus; E-mail: jzus@ Science Letters: Functional analysis of helicase and three tandem HRDC domains of RecQ in Deinococcus radiodurans* HUANG Li-fen1,2, HUA Xiao-ting1, LU Hui-ming1, GAO Guan-jun1, TIAN Bing1, SHEN Bing-hui3, HUA Yue-jin??1 (1Institute of Nuclear-Agricultural Science, Zhejiang University, Hangzhou 310029, China) (2Jiangsu Province Key Lab of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China) (3Division of Molecular Biology, City of Hope National Medical Center and Beckman Research Institute, Duarte, CA 91010-3000, USA) ?E-mail: yjhua@ Received Mar. 27, 2006; revision accepted Mar. 31, 2006 Abstract: RecQ is a highly conserved helicase necessary for maintaining genome stability in all organisms. Genome comparison showed that a homologue of RecQ in Deinococcus radiodurans designated as DR1289 is a member of RecQ family with unusual domain arrangement: a helicase domain, an RecQ C-terminal domain, and surprisingly three HRDC domain repeats, whose func- tion, however, remains obscure currently. Using an insertion deletion, we discovered that the DRRecQ mutation causes an increase in gamma radiation, hydroxyurea and mitomycine C and UV sensitivity. Using the shuttle plasmid pRADK, we complemented various domains of the D. radiodurans RecQ (DRRecQ) to the mutan