紫外光谱分析-演讲选编.ppt

Ultraviolet/Visible spectroscopy Fifth group speaker:Gao xianzhe UV radiation UV absorption 日常生活中, 各种溶液之所以呈现不同的颜色, 与它对光的选择性吸收有关。当一束由各种波长按一定比例组成的光透过一溶液时, 某些波长的光被溶液吸收, 而另一些波长的光不被吸收而透过溶液。当透过的光包含有可见光波长范围内的光时, 就可以被人眼察到, 溶液的颜色正是由透过光的波长所决定。 UV/Vis spectroscopy Definition: It’s the analysis methods with analysis,determination and inference of material composition,content and structure by using he uv-vis spectra and the degree of absorption which produced by material molecules, or ions on the absorption of ultraviolet and visible light. Features: high sensitivity, high accuracy, good selectivity, easy to operate, fast analysis speed, wide application range. Absorption curve General components (Ⅱ) monochromator Monochromator is the device required monochromatic light from the continuous spectrum.prism and grating monochromator are the most common Monochromator. The shortcomings of prism monochromator is that dispersion rate varies with wavelength,the spectra obtained were non uniformly arranged,and the light transfer efficiency is low. grating monochromator has a good and the same dispersion capacity almost in the whole spectral region . Therefore, the modern ultraviolet visible spectrophotometer using grating monochromator. (Ⅲ) sample room The sample chamber is placed in a variety of types of absorption pool (the ratio of the colored dishes) and the corresponding tank rack accessories. There are two main types of the absorption pool, quartz and glass.Commonly,we should use the quartz pool in the UV visible region,and use the glass tank in the visible region . （四）检测器 利用光电效应将透过吸收池的光信号变成可测的电信号。常用的检测器有光电池、光电管和光电倍增管。 二、仪器的类型 （一）Single-beam UV-Vis spectrophotometer （三）Double-wavelength 一个光源，两个单色器，一个吸收池 Application of ultraviolet absorption spectrum The applicability