2004小麦抗旱基因CAT的表达5.24.pdf

Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat Celina M. Luna*, Gabriela M. Pastori, Simon Driscoll, Karin Groten, Stephanie Bernard and Christine H. Foyer? Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK Received 27 May 2004; Accepted 24 September 2004 Abstract Plants co-ordinate information derived from many di- verse external and internal signals to ensure appropri- ate control of gene expression under optimal and stress conditions. In this work, the relationships be- tween catalase (CAT) and H2O2 during drought in wheat (Triticum aestivum L.) are studied. Drought- induced H2O2 accumulation correlated with decreases in soil water content and CO2 assimilation. Leaf H2O2 content increased even though total CAT activity doubled under severe drought conditions. Diurnal regu- lation of CAT1 and CAT2 mRNA abundance was ap- parent in all conditions and day/night CAT1 and CAT2 expression patterns were modified by mild and severe drought. The abundance of CAT1 transcripts was regulated by circadian controls that persisted in con- tinuous darkness, while CAT2 was modulated by light. Drought decreased abundance, and modified the pat- tern, of CAT1 and CAT2 mRNAs. It was concluded that the complex regulation of CAT mRNA, particularly at the level of translation, allows precise control of leaf H2O2 accumulation. Key words: Catalase, diurnal cycle, drought, hydrogen peroxide, Triticum aestivum L. Introduction Drought stress is a complex syndrome involving not only water deprivation but also nutrient limitation, salinity, and oxidative stresses. Moreover, levels of light that are optimal for photosynthesis in well-watered plants become excessive in plants suffering water deprivation. Photosynthesis is particularly sensitive to water deficit because the stomata close to conserve water as available soil water declines. Stomatal closure deprives the leaves of carbon dioxide and photosynth