BIOTECHNOLOGY IN CROP PROTECTION TOWARDS SUSTAINABLE INSECT CONTROL.pdf

收稿日期：2009-05-22；接受日期：2009-07-16 基金项目：国家重点基础研究发展计划(2007CB411600)；全球环境基金会/世界银行项目(GEF-MSP grant No. TF051795)；云南省发展和改 革委员会项目；中国科学院西部之光项目(292006312D11033)；云南省科技厅社会发展科技计划(2008CA001) *通讯作者（Corresponding author），E-mail: yangjx@mail.kiz.ac.cn 动 物 学 研 究 2009，Aug. 30(4):463-467 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI：10.3724/SP.J.1141.2009.04463 抚仙金线鲃人工繁殖与鱼苗培育技术 潘晓赋，刘淑伟，李再云，杨君兴* （中国科学院昆明动物研究所，云南 昆明 650223） 摘要：抚仙金线鲃(Sinocyclocheilus tingi)是抚仙湖的特有种。2009年 3－4月，有 4次繁殖试验取得了成功。 先后试验了 40尾雌鱼和 20尾雄鱼，成功催产 19尾雌鱼和 15尾雄鱼，分别占雌雄鱼的 47.5%和 75.0%。获得鱼 卵 25 547粒，并实施干法受精。有 16 810粒受精，平均受精率为 65.8%。孵化出鱼苗约 6 040尾，平均孵化率为 23.6%。经过 20 日的饲养，仔鱼存活 3 056 尾，存活率为 50.6%。抚仙金线鲃较低的催产率，可能是生殖功能紊 乱所致；但也不排除是人工营造的条件离其自然环境需求尚有距离。要使抚仙金线鲃成为鱼康鱼良白鱼(Anabarilius grahami)之后又一当地特色养殖鱼种，突破其人工繁殖瓶颈，培育高品质鱼苗是必由之路。突破其人工繁殖，还 可为研究抚仙金线鲃的生活史和保护该物种免于灭绝奠定良好基础。 关键词：抚仙金线鲃；人工繁殖；鱼苗培育；抚仙湖 中图分类号：Q959.468；S962 文献标识码：A 文章编号：0254-5853-(2009)04-0463-05 Artificial Propagation and Larvae Cultivation of Sinocyclocheilus tingi PAN Xiao-fu, LIU Shu-wei, LI Zai-yun, YANG Jun-xing* (Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China) Abstract: The Fuxian golden line (Sinocyclocheilus tingi) is a famous fish endemic to Fuxian Lake. From March to April 2009, four breeding experiments were undertaken. 40 females and 20 males were injected with hormones. 19 females and 15 males bred successfully, accounting for 47.5% of females and 75.0% of males. A total of 25 547 eggs were obtained and subjected to dry-fertilization, with 16 810 eggs being fertilized with a 65.8% success rate. 6 040 larvae were obtained with a 23.6% hatching rate. 3 056 larvae survived 20 days of cultivation with a 50.6% survival rate. Reproductive dysfunctions are major constraining factors to low Induced spawning of S. tingi in a captive environment. Artificial conditions that did not reflect the necessary natural env