Bone tissue engineering with premineralized silk scaffolds.pdf

Bone 42 (2008) 1226–1234 Contents lists available at ScienceDirect Bone j ourna l homepage: www.e lsev ie / locate /boneBone tissue engineering with premineralized silk scaffolds Hyeon Joo Kim a,b,d, Ung-Jin Kim a,b,d,e, Hyun Suk Kim a,b,d,f, Chunmei Li a,b,d, Masahisa Wada g, Gary G. Leisk c, David L. Kaplan a,b,d,? a Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA b Department of Chemical and Biological Engineering, Tufts University, Medford, MA 02155, USA c Department Mechanical Engineering, Tufts University, Medford, MA 02155, USA d Bioengineering and Biotechnology Center, Tufts University, Medford, MA 02155, USA e Mecellose RD Team, RD Center, Samsung Fine Chemicals Co., Ltd., 103-1 Munji-dong, Yusung-gu, Taejeon 305-380, Republic of Korea f Department of Polymer Science and Engineering, Inha University, Incheon 402-751, Republic of Korea g Department of Biomaterials Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japana r t i c l e i n f o? Corresponding author. Department of Biomedical Medford, Massachusetts 02155, USA. Fax: +1 617 627 32 E-mail address: david.kaplan@ (D.L. Kaplan 8756-3282/$ – see front matter ? 2008 Elsevier Inc. Al doi:10.1016/j.bone.2008.02.007a b s t r a c tArticle history: Received 26 January 2007 Revised 3 February 2008 Accepted 7 February 2008 Available online 4 March 2008Edited by: S. Goldstein 2 2 4 composite scaffolds were subsequently seeded with human bone marrow stem cells (hMSC) and cultured inSilk fibroin biomaterials are being explored as novel protein-based systems for cell and tissue culture. In the present study, biomimetic growth of calcium phosphate on porous silk fibroin polymeric scaffolds was explored to generate organic/inorganic composites as scaffolds for bone tissue engineering. Aqueous-derived silk fibroin scaffolds were prepared with the addition of polyaspartic acid during processing, followed by the cont