Differentiation of three pairs of aconite alkaloid isomers from Aconitumnagarumvarlasiandrum by EIMS.pdf

下载文档

6
0
约4.02万字
约 14页
2017-04-13 发布于江苏
举报
版权申诉
保障服务

Differentiation of three pairs of aconite alkaloid isomers from Aconitumnagarumvarlasiandrum by EIMS.pdf

1、本文档共14页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

Differentiation of three pairs of aconite alkaloid isomers from Aconitumnagarumvarlasiandrum by EIMS

RAPID COMMUNICATIONS IN MASS SPECTROMETRY Rapid Commun. Mass Spectrom. 2006; 20: 157–170 Published online in Wiley InterScience (). DOI: 10.1002/rcm.2283 Differentiation of three pairs of aconite alkaloid isomers from Aconitum nagarum var. lasiandrum by electrospray ionization tandem mass spectrometry Rui Li, Zhijun Wu, Fan Zhang and Lisheng Ding* Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China Received 18 August 2005; Revised 31 October 2005; Accepted 7 November 2005 Three pairs of isomers of aconite alkaloids from Aconitum nagarum var. lasiandrum have been investigated by electrospray ionization mass spectrometry (ESI-MS) and tandemmass spectrometry (MS/MS) employing ion-trap and quadrupole time-of-flight mass spectrometers in positive mode. Based on the differences of their fragmentation pathways and special fragment ions, three pairs of isomers of aconite alkaloids were differentiated. In addition, fragmentation laws of some veatch- ines and the discrepancy of fragmentation mechanisms between veatchine-type and aconitine-type alkaloid were also concluded. In the case of veatchines, a radical would be formed by homolysis of C18–C4 or C18–H bonds, followed by elimination of a series of C2H2 and C2H4. Moreover, the retro-Diels-Alder (RDA) reaction occurred in the E-ring and double-electron transfer triggered by the positive charge on C1 led to the formation of diagnostic ions at m/z 216. With regard to aconitine-type alkaloids, the N-substituent is not eliminated easily. Although there is no carbonyl group on some aconitine-type alkaloids, with hydroxyl and methoxyl on C15 and C16 respectively, CO was readily eliminated through tautomerization. Copyright # 2005 John Wiley Sons, Ltd. Aconitum nagarum var. lasiandrum (Ranuculaceae) is mainly distributed in the Yunnan Province of China. Its roots can relieve pain and dispel dampness. The roots of this species have been used as a folk medicine in China for the treatment of cold limb