Ginkgo Biloba Extract.pdf

ISSN: 1524-4628 Copyright ? 2008 American Heart Association. All rights reserved. Print ISSN: 0039-2499. Online Stroke is published by the American Heart Association. 7272 Greenville Avenue, Dallas, TX 72514 DOI: 10.1161/STROKEAHA.108.523480 2008;39;3389-3396; originally published online Oct 9, 2008; Stroke Sofiyan Saleem, Hean Zhuang, Shyam Biswal, Yves Christen and Sylvain Doré 1 in Ischemic Reperfusion Brain Injury Ginkgo Biloba Extract Neuroprotective Action Is Dependent on Heme Oxygenase /cgi/content/full/39/12/3389 located on the World Wide Web at: The online version of this article, along with updated information and services, is /reprints Reprints: Information about reprints can be found online at journalpermissions@ 410-528-8550. E-mail: Fax:Kluwer Health, 351 West Camden Street, Baltimore, MD 21202-2436. Phone: 410-528-4050. Permissions: Permissions Rights Desk, Lippincott Williams Wilkins, a division of Wolters /subscriptions/ Subscriptions: Information about subscribing to Stroke is online at by on June 8, 2009 Downloaded from Ginkgo Biloba Extract Neuroprotective Action Is Dependent on Heme Oxygenase 1 in Ischemic Reperfusion Brain Injury Sofiyan Saleem, PhD; Hean Zhuang, MD; Shyam Biswal, PhD; Yves Christen, PhD; Sylvain Dore?, PhD Background and Purpose—Ginkgo biloba extracts are now prescribed in several countries for their reported health benefits, particularly for medicinal properties in the brain. The standardized Ginkgo extract, EGb761, has been reported to protect neurons against oxidative stress, but the underlying mechanisms are not fully understood. Methods—To characterize the oral consumption of EGb761 in transient ischemia, we performed the middle cerebral artery occlusion (MCAO) filament model in wild-type and heme oxygenase 1 (HO-1) knockouts. Mice were pretreated for 7 days before the transient occlusion or posttreated acutely during reperfusion; then neurobehavioral scores and infarct volumes were assessed. Furth