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Factors affecting selective rejection of proteins within a binary mixture during cross-flow ultrafil.pdf

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Factors affecting selective rejection of proteins within a binary mixture during cross-flow ultrafil

Ai c I i s T m t ? K 1 b c t a d H l m t o a a O c 0 dJournal of Membrane Science 281 (2006) 103–110 Factors affecting selective rejection of proteins within a binary mixture during cross-flow ultrafiltration Min-Yu Teng a, Su-Hsia Lin a,c,?, Chung-Yi Wu a, Ruey-Shin Juang b a Department of Chemical Engineering, Nanya Institute of Technology, Chung-Li 320, Taiwan b Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li 320, Taiwan c R D Center for Membrane Technology, Chung Yuan University, Chung Li, Taoyuan 32033, Taiwan Received 6 October 2005; received in revised form 12 March 2006; accepted 14 March 2006 Available online 24 March 2006 bstract The flux decline and rejection behavior in cross-flow ultrafiltration (UF) of BSA/lysozyme (Ly) mixtures, with and without ultrasound, were nvestigated in upward and downward modes. Polysulfone (PS) and polyvinylidine fluoride (PVDF) ultrafilters were selected. Experiments were onducted at different pH values (4.9–11), applied pressures (2–4 atm), added NaCl concentrations (0.01–1 g/L), and ultrasonic powers (180–250 W). t was shown that PS membrane yielded higher flux and lower Ly rejection than PVDF membrane. Low rejection of Ly was achieved at pH near soelectric point of Ly (11.0), particularly under the conditions of lower applied pressures and ionic strengths. The flux and Ly rejection were trongly affected by the solution environment. BSA was almost retained in the retentate, but its existence had a significant effect on permeate flux. he flux was enhanced with ultrasound and the enhancement increased with increasing ultrasonic power at 25 kHz, likely due to the change of olecular sizes of the proteins under ultrasonic irradiation. With ultrasound, the level of flux enhancement in the upward mode was slightly better han that in the downward mode. 2006 Elsevier B.V. All rights reserved. ; Flux t t t s m t h l p t s ( teywords: Cross-flow ultrafiltration; Protein mixtures; Ultrasound; Se