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High-resolution genetic map of the Rvi15 (Vr2) apple scab resistance
High-resolution genetic map of the Rvi15 (Vr2) apple scab
resistance locus
Paolo Galli ? Giovanni Antonio Lodovico Broggini ?
Markus Kellerhals ? Cesare Gessler ?
Andrea Patocchi
Received: 20 July 2009 / Accepted: 11 January 2010 / Published online: 28 January 2010
Springer Science+Business Media B.V. 2010
Abstract The Rvi15 (Vr2) apple scab resistance
locus found in the GMAL 2473 accession has been
previously mapped to the top of the Linkage Group 2
(LG2) by analyzing 89 progeny plants of a cross
between ‘Idared’ and GMAL 2473. A new population
of 989 progeny plants, derived from a cross between
‘Golden Delicious’ and GMAL 2473, has been
analyzed with the two SSR markers CH02c02a and
CH02f06, previously found to be associated with
Rvi15 (Vr2), and with two published markers derived
from NBS sequences (ARGH17 and ARGH37)
estimated to map close to the Rvi15 (Vr2) locus.
ARGH17 and ARGH37, were found to be the closest
markers to the resistance locus, bracketing it within
an interval of 1.5 cM. The SSRs mapped one on each
side of Rvi15 (Vr2). CH02f06 mapped at 2.9 cM from
ARGH37 while CH02a02a mapped at 1.7 from
ARGH17. The position of Rvi15 (Vr2) respect to
CH02a02a indicates that Rvi15 (Vr2) and Rvi4 (Vh4),
a second apple scab gene mapped on the top of LG2,
are two different resistance genes. In order to develop
even more tightly linked markers to Rvi15 (Vr2),
ARGH17 was used as the starting point for chromo-
some walking through the Rvi15 (Vr2) homolog
region of the cv. ‘Florina’. A single ‘Florina’ BAC
clone, 36I17, was sufficient to span the homologous
locus in the new population’s recombinant progeny.
Sequencing of the 36I17 BAC clone allowed identi-
fying seven putative ORFs, including two showing a
TIR-NBS-LRR structure. Ten additional markers
could be developed mapping within a 1.8 cM interval
around the Rvi15 (Vr2) resistance gene. ARGH17 and
GmTNL1 markers, the latter also derived from NBS-
LRR resistance gene homolog sequence, are the
closest markers to R
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