ITIS MyGo Pro Performance Data.pdfVIP

MyGo Pro? System Performance Data The MyGo Pro? Instrument looks great on your bench - and produces beautiful data. Like all of our IT-IS Life Science PCR systems, the MyGo Pro? System uses excellent thermal control for high reproducibility of results obtained with the 32 samples tested in the same run. High resolution for copy number determination and melting curve analysis is achieved with great speed. Here is a list of examples to illustrate these performance features. Click the ones that are most relevant for your application, and enjoy the data (of course other reagents may produce these results also, you are not limited to the ones listed below) - Topic Template Detection Format Reagent Dynamic range of quantification (2-fold dilutions) plasmid DNA Universal ProbeLibrary probe FastStart Essential DNA Probes Master Dynamic range of quantification (10-fold dilutions) plasmid DNA Universal ProbeLibrary probe FastStart Essential DNA Probes Master Reproducibility of Cq values human genomic DNA hydrolysis probe FastStart Essential DNAProbes Master Endpoint Genotyping DNA hydrolysis probe FastStart Essential DNAProbes Master High Resolution Melting DNA ResoLight dye LightCycler? 480 HighResolution Melting Master Absolute Quantification DNA SYBR Green I FastStart Essential DNAGreen Master Homogeneity of Tm values human genomic DNA SYBR Green I FastStart Essential DNAGreen Master Observation Result The MyGo Pro Instrument reliably discriminates template dilutions, even differences by a factor of 2, producing reproducible replicate Cq values. Serial amplification plots of 2-fold dilutions show the high resolution detection and differentiation. Dynamic range of quantification (2-fold dilutions) Purpose of the experiment Dynamic resolution of gene quantification using Universal ProbeLibrary probes. Procedure Real-Time PCR using 2-fold dilutions of plasmid DNA (starting from one million copies) as template, and a Universal ProbeLibrary probe for product detection. Pu