Q_Exactive培训教程20150902.pdf

Q Exactive Operations Training Course Thermo Fisher Scientific 1 Course Outline ? Chapter 1: Mass Spectrometry Basic ? Chapter 2: Notes on Liquid Chromatography ? Chapter 3: Ions Optics and Mass Filter ? Chapter 4: Ion Detection-Orbitrap ? Chapter 5: Scan Modes on the Q Exactive ? Chapter 6: Tune and Calibration 2 Practical ? Tuning and calibration ? ESI compound optimization ? Instrument Method development-Xcalibur software ? Setting and running a sequence ? Data analysis of LC-MS data using Xcalibur ? Instrument maintenance 3 Chapter 1 The world leader in serving science Mass Spectrometry Basic Q Exactive 仪器前视图 5 What is Mass Spectrometry? “The basis in MS (mass spectrometry) is the production of ions, that are subsequently separated or filtered according to their mass-to-charge (m/z) ratio and detected. The resulting mass spectrum is a plot of the (relative) abundance of the produced ions as a function of the m/z ratio.” Niessen, W. M. A.; Van der Greef, J., Liquid Chromatography–Mass Spectrometry: Principles and Applications, 1992, Marcel Dekker, Inc., New York, p. 29. data_dependent_01 #634-649 RT: 19.84-20.25 AV: 10 NL: 4.02E5 F: + c Full ms [150.00-1000.00] 750.42 100 90 80 70 60 50 40 RelativeAbundance 181.05 30 20 208.05 333.03 10 732.64 806.20 226.08 370.50 403.02 524.60 560.67 663.90 714.58 828.26 922.07 974.26 0