分光光度法测定痕量锌的方法研究_学位论文.doc

分光光度法测定痕量锌的方法研究 [摘要]自八十年代以来，环境分析尤其是水体中水溶态金属子的分析的研究在国内外都很活跃。分光光度法是测定水溶态微量金属离子的常用检测方法，该方法操作简便，仪器结构简单、价格低廉。双水相萃取技术是二十世纪五十年代发展起来的新型分离技术。它具有以水为溶剂、价格低廉、不易挥发、污染小、分相速度快、操作简单、可以与其他技术相结合等优点，已经得到业界的广泛重视。通过双水相萃取技术与分光光度法集成应用，建立了集萃取分离和测定金属离子于一身的双水相萃取光度分析方法。 系统地研究了锌(11)一锌试剂的配合物在聚乙二醇 (PEG)2000一硫酸钠双水相体系中的光谱性质、组成、显色和萃取条件，建立了分析锌(II)的新方法。通过对PEG溶液用量、硫酸钠用量、显色剂的用量、体系的酸度等影响因素的优化，提高了选择性和灵敏度、基本消除了基体干扰。从线性关系、检出限、精密度及抗干扰能力等方面对该方法进行评价。将该方法用于食品等实际样品的分析测定，结果与原子吸收光谱法测定值相符。 [关键词]：聚乙二醇；双水相萃取；分光光度法；锌( = 2 \* ROMAN II) [Abstract]The studies of environmental analysis， especially the analysis of water soluble metalioninwater， are active worldwide from 1980，s. Spectrophotometry is the most popular deteetion method applied to determine water soluble trace metal ion.It is simple and convenient， and its instrument is also simple and low in price.Aqueous two-phase extraction technique， as a new type of separating technique developed from 1950，s， has reeeived broad attention in extraction area， because of its advantages，such as， water as solvent， low cost， less volatile， less pollution， fast phase se parating speed， simple operation，combination with other techniques. By the integrated applieation of aqueous two-phase extraction and spectrophotometry， the novel analysis method that aqueous two- phase extraction spectrophotometry was established.It can extract and simultaneously determine metal ions. The spectral properties，composition， color and extraetions conditions of zine( = 2 \* ROMAN II)- zineon eomplexes in the aqueous two-phase system formed by polyethylene glyeol(PEG) 2000 and sodium sulfate were systematieally studied， and the novel method of analyzing zinc( = 2 \* ROMAN II)is established. The seleetivity and sensitivity was improved， and the matrix interference was basieally eliminated by optimizing the amount of PEG solution， sodium sulfate，chromogenie reagent， the acidity of the system and other，factors. The method was evaluated from linear relationship， deteetion limits P