基于脑缺血动物模型的针刺作用机制及选穴规律的挖掘研究.PDFVIP

国际中医中药杂志2017年4月第39卷第4期 Int J Trad Chin Med, April 2017, Vol. 39, No.4 ·333· ·论著· 基于脑缺血动物模型的针刺作用机制 及选穴规律的挖掘研究 黄满婷 雷蕾 李海燕 【摘要】 目的 运用数据挖掘方法分析针刺实验文献中针刺对脑缺血动物模型的分子生物学作用 机制的选穴规律。方法 检索 1978 年 1 月－2015 年 12 月中国期刊全文数据库(CNKI)、中国生物医学文 献数据库(SinoMed)、万方科技数据库有关针刺动物实验的文献，筛选出与针刺对脑缺血动物模型基因蛋 白表达影响相关的文献，人工提取文献中的穴位和基因蛋白指标，规范化处理后进行频次统计、关联规 则及复杂网络计算分析并可视化展示。结果 获得符合研究范围的文献 350 篇；获得穴位 79 个，频次最 高为百会；获得基因蛋白 180 个，频次最高为 BCL2；关联规则计算得最常用组穴为足三里、曲池；复杂 网络计算得与脑缺血损伤或康复机制关系较密切的基因/蛋白为热休克蛋白 70、BCL2、CASP3、BCL2 相 关 X 蛋白。结论 针刺穴位可通过多条途径治疗缺血性脑血管疾病，穴位选择主要以督脉和阳明经的穴 位为主。 【关键词】 脑缺血；针刺穴位；基因表达；复杂网络；关联规则 Tentative exploration of acupoints’ biology effects to the cerebral ischemia model Huang Manting, Lei Lei, Li Haiyan. Institute of Information on Traditional Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China Corresponding author: Li Haiyan, Email: li_haiyan@126.com 【Abstract】 Objective The molecular biology mechanism of cerebral ischemia animal models be analyzed from acupuncture experimental literatures by using data mining methods as a tentative exploration. Methods Retrieve the literatures of acupuncture animal experiments during 1978-2015, and screen the literatures about acupuncture effect on cerebral ischemia animal model of gene protein expression. Acupoint and gene/protein targets were extracted and normalized, and then the frequency statistics, association rule, calculation of complex networks were analyzed and visually displayed. Results A total of 350 papers were included. Seventy-nine acupoints were summarized, and the most frequent acupoint was baihui; A total of 180 genes/proteins were found, and the most frequent genes/proteins was BCL2. The most common group acuppoi