33连亨宁结核和非小细胞肺癌患者胸腔积液的蛋白质组学研究.docx

结核和非小细胞肺癌患者胸腔积液的蛋白质组学研究 连亨宁，李园园，贾罄竹，但小苹，熊玮，万瑛* [摘要] 目的：通过比较结核性胸腔积液和非小细胞肺癌患者的胸腔积液蛋白质组成差异，寻找潜在鉴别诊断标志蛋白质。方法：收集结核性胸腔积液样本和非小细胞肺癌胸腔积液样本，使用聚丙烯酰胺凝胶电泳—纳升液相色谱—超高分辨飞行时间质谱进行蛋白质鉴定。结果：结核性胸腔积液中共检测出人类蛋白质517种和233种结核菌蛋白质，肺癌胸腔积液共检测出人类蛋白质375种。蛋白功能分析显示结核性胸腔积液中细胞周期和细胞运动的蛋白质富集明显，而肺癌胸腔积液中Ras信号转导和DNA甲基化的蛋白质富集明显。其中白介素Iα（IL1A）仅存在于肺癌胸腔积液中，结核菌大膜蛋白1（Mmpl1）和结核菌蛋白质Rv2567仅存在于结核性胸腔积液样本中。结论：IL1A、Mmpl1、Rv2567可能成为鉴别结核性胸腔积液和肺癌???腔积液的潜在标志蛋白。 【关键词】 胸腔积液；结核；非小细胞肺癌；蛋白质组学；质谱 【中图分类号】R392-33 【文献标识码】A Proteomic Profiling of Pleural Effusion in tuberculosis and lung cancer patients Lian Hengning1 , Li Yuanyuan1 , Jia Qingzhu1 , Dan Xiaoping2 , Xiong Wei2 , Wan Ying1,* , 1. Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China; 2. Department of Respiratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China *Corresponding Author: Wan Ying, E-mail: wanying@ [Abstract]：The proteome of pleural effusion from lung adenocarcinoma and tuberculosis (TB) patients was investigated by using SDSand Nano-HPLC-ESI-QTOF-MS/MS platform in series, to find potential diagnostic protein markers for lung cancer and TB pleural effusion. The pleural effusion from TB and non-small-cell lung cancer (NSCLC) patients was collected. 517 human proteins and 233 TB proteins were identified in the TB pleural effusion, in which the proteins participated cell cycle and cell motion were enriched. 375 human proteins were identified in the NSCLC pleural effusion, in which the proteins participated the regulation of Ras signal transduction were enriched. Interleukin-1 alpha (IL1A) was detected in all NSCLC group but not in the TB group. Mycobacterial membrane protein Large 1 (Mmpl1) and Rv2567 (an Uncharacterized TB protein) was detected only in TB group, but not in NSCLC group. Thus our study suggested that, IL1A, Mmpl1, Rv2567 might be the potential diagnostic marker of the TB and NSCLC, which need to be validated in clinical. [Key words] Pleural effusion; t