分子遗传学8课件.pptVIP

Chapter 3;Key Points;Bases;Nucleosides;Nucleotides;DNA Double Helix;Different Forms of DNA;Circular DNA Molecules;High-Order Structure of RNA;Modified Nucleic Acids;Stability of Nucleic Acids;Effect of Acid on Nucleic Acids;Effect of Alkali on Nucleic Acids ;High axial ration: 2 nm in diameter/centimeters in length Relatively stiff: high viscosity Problems: easily damaged by shearing forces or sonication and thus strong shearing forces such as over-pipetting and vortexing should be avoided for preparation of high quality (high molecular) genomic DNA and supercoiled plasmid DNA Applications: DN