基于SRAP的叶子花种质资源遗传多样性及遗传关系分析.pdfVIP

热带亚热带植物学报　2014， 22(2)： 147 ~ 154 Journal of Tropical and Subtropical Botany 基于SRAP的叶子花种质资源遗传多样性及遗传 关系分析 唐源江*, 武晓燕, 曹雯静 (华侨大学花卉工程研究所，福建 厦门 361021) 摘要： 为探讨叶子花(Bougainvillea sp.)品种间的遗传关系，应用 SRAP (Sequence-related amplified polymorphism)标记技术对 48 个叶子花品种的遗传多样性及遗传关系进行了分析。结果表明，从 208 对引物中筛选出 25 对多态性较高的引物组合，共扩增 出 773 条清晰条带，其中多态性条带 750 条，平均多态性条带百分率达 97.02%。UPGMA 聚类分析结果表明，48 个叶子花品 种的遗传相似性系数为 0.4058 ~ 0.8568，在遗传相似性系数 0.558 水平上，可分为 4 个类群，福摩萨叶子花与毛叶紫花叶子花 各自成一类，其它品种分为两大类群。SRAP 标记可较好地反映叶子花种质间的遗传关系，为合理利用叶子花种质资源及提高 育种效率提供了科学基础。 关键词： 叶子花； 种质资源； 品种； 遗传多样性； SRAP doi: 10.3969/j.issn.1005–3395.2014.02.007 Genetic Diversity and Relationship of Bougainvillea Germplasm Resources Based on SRAP Markers TANG Yuan-jiang*, WU Xiao-yan, CAO Wen-jing (Institute of Flower Engineering, Huaqiao University, Xiamen 361021, China) Abstract: In order to understand the genetic relationship of Bougainvillea germplasms, the SRAP (sequence-related amplified polymorphism) markers were firstly applied on analysis of genetic diversity and relationship among 48 Bougainvillea cultivars. The results showed that twenty-five primer pairs screened from 208 primer pairs amplified a total of 773 bands, of which 750 were polymorphic bands, and the percentage of polymorphic bands was 97.02%. The genetic similar coefficient of Bougainvillea germplasms ranged from 0.4058 to 0.8568, and UPGMA (Unweighted pair-group method arithmetic average) analysis showed that 48 cultivars could clustered into 4 groups with the genetic similarity coefficient of 0.558. However, the cultivar B. glabra ‘Formosa’ and B. spectabilis ‘Spectabilis’ were clustered into a separate cluster, respectively, the other cultivars were clustered into two groups. The genetic relationships of all cultivars examined would be made clear based on SRAP molecular markers. The results could provide scientific foundation for using germplasm resource appropriately and improving the effi