铁、钙离子补料对杜氏盐藻生长的影响.pdfVIP

研究报告 中 国 酿 造 2014年第33卷第11期 总第273期 ·51· 铁、钙离子补料对杜氏盐藻生长的影响 吕和鑫，贾士儒 ，崔相敢，夏 锋 (天津科技大学 天津市工业微生物重点实验室，天津 300457) 摘 要：通过原子吸收光谱测定了杜氏盐藻对钙、镁、钾、铁四种离子的消耗速率，构建金属离子含量限制型培养基，研究了补加金属 离子对杜氏盐藻生长光合速率、色素含量的影响。结果表明，对数期杜氏盐藻细胞对铁离子与钙离子的消耗呈线性降低，每增殖lO 个细胞分别消耗0．042 g的铁离子与0．708 Ixg的钙离子，而在稳定期后，钙、铁离子浓度继续下降。生长时期中钾离子与镁离子的含 量变化不显著。铁、钙离子补料能恢复杜氏盐藻的生长，但光合活性与色素含量变化较补料前的对数期光合速率与色素含量要低，但 总类胡萝 h素含量要高于补料前对数期的。培养到稳定期的培养基经过回收补加有限的铁、钙离子后，细胞生长延滞期消失，但稳定 期细胞浓度降低。回流培养基对数期细胞的光合速率与叶绿素含量降低，但是总类胡萝 卜素含量升高。 关键词：杜氏盐藻；口．胡萝 卜素；金属离子；消耗速率；基质消耗动力学 中图分类号：Q939．1 文献标识码：A 文章编号：0254—5071(2014)11—0051—04 doi：l0．118820．issn．0254-5071．2014．11．011 Efects ofthe fed-batchcultivationofmineralnutrients onDunaliclla salinagrowth Lt3 Hexin，JIA Shiru ，CUI Xianggan，XIA Feng (TianjinKeyLaboratoryoflndustnalMicrobiology,Tianjin UniversityofScienceTechnology,Tianjin300457,China) Abstract：The consumption rate of DunMidla salina on iron，potassium，calcium and magnesium were determined by atomic absorption spectrum． The metal ion content limit type medium was established and the efect ofadding metal ion on D．salina growth，photosynthetic rate and pigment con- tent were studied．The results showed that the consumption of iron and calcium decreased linearly during the log growth phase．Th e consum ption rates of iron an d calcium in the medium were 0．042 g and 0．708 g for 10 cel proliferation，and after stable phase，calcium，iron concentration continued to fal1．However,the diference of potassium and magn esium concentration in the medium was not signifcant in the growth period．The feeding of iron and calcium ions could promote the growth of D．sa／na cels to revive but with a lower photosynthetic rates an d chlorophyl conten~， the total carotenoid content was higher than logarithmic phase before feeding．Alter recycling adding limited iron and calcium to the medium in the stable phase，the cell lag phase disappeared but the cel concentration decreased in the stable phase．Th e photosyn thetic rate and chlorophyll content ofcel in log phase in