混合菌群DDMY1对活性黑5的脱色降解研究.PDFVIP

第9 卷 第24 期 Vo l.9 No .2 4 2016 年 12 月 December 2016 混合菌群 DDMY1 对活性黑 5 的脱色降解研究 谢学辉* （东华大学环境科学与工程学院，上海 201620） 摘要：利用梯度浓度压力驯化法，从运行良好的印染废水水解酸化反应器中筛选出对染料活性黑 5 具有良好脱 色性能的混合菌群 DDMY1，利用该菌群在兼氧条件下对活性黑 5 进行脱色研究。研究表明，混合菌群 DDMY1 对活性黑 5 的脱色性能显著，400 mg/L 活性黑 5 染料经过 24 h 培养，脱色率能达到 97.4%. 运用紫外?可见全 波长扫描分光光度法检测不同时间、不同状态下脱色液吸光值情况。运用气相色谱?质谱联用（gas chromatography-mass spectrometer，GC-MS）和傅里叶转变红外光谱（Fourier transform infrared spectroscopy， FTIR）等检测方法分析生物脱色过程中的产物情况，检测到含有芳环或酰胺结构的物质可能是活性黑 5 的生 物降解产物，从染料结构变化的角度揭示活性黑 5 生物脱色降解过程。 关键词：环境生物学；混合菌群；染料脱色；染料降解；活性黑 5 中图分类号：X172 文献标识码：A 文章编号：1674-2850(2016)24-2564-08 Decolorization and degradation of reactive black 5 by mixed flora DDMY1 XIE Xuehui (College of Environmental Science Engineering, Donghua University, Shanghai 201620, China) Abstract: In this paper, by using the concentration gradient pressure screening method, a mixed bacterial flora DDMY1, which has good decolorization performance on reactive black 5, was selected from the well-runnig hydrolytic acidification reactor for printing and dyeing wastewater treatment. The bacterial flora was used to decolor reactive black 5 under the facultative-aerobic condition. Results showed that the mixed flora DDMY1 performed significantly in decoloring of reactive black 5, with 400 mg/L reactive black 5 for 24 h cultivation, the decolorization rate could reach up to 97.4%. Using ultraviolet-visible spectrophotometry method scanned the decoloring liquid at different time and different states. Using gas chromatography-m