(CANCERRESEARCH36,3608-3615,.PDFVIP

(CANCERRESEARCH36,3608-3615,October1976] Tumoricidal Properties of Mouse Macrophages Activated with Mediators from Rat Lymphocytes Stimulated with Concanavalin A1 I. J. Fidler, J. H. Darnell, and M. B. Budmen Basic Research Program, National Cancer Institute, Frederick Cancer Research Center, Frederick, Maryland 21701 SUMMARY tered properties, including increased phagocytosis, higher degradative enzyme activities, increased cytostatic capabil Macrophage-activating factor (MAF) was obtained from ity (8), increased tumoricidal properties (4, 14, 15, 34), and cultures of normal F344 rat lymphocytes incubated with increased specific a€?arminga€?ofmacrophages (12, 17, 32). insoluble concanavalin A. The MAF rendered macrophages We have reported that macrophages collected from nor from normal CS7BL/6 mice cytotoxic against the syngeneic mal mice or mice bearing progressively growing tumor were B16 melanoma and the allogeneic AC 15091. At the same not cytotoxic in vitro to their syngen??ictumor cells. How time, normal syngeneic or allogeneic embryo cells were ever, these macrophages were potentially cytotoxic to the unharmed, even in the presence of susceptible tumor cells. syngeneic tumor cells after activation in vitro by MAFs Optimal MAF levels followed incubation of lymphocytes for released from sensitized syngeneic, allogeneic and xenoge 48 hr with Sepharose-bound concanavalin A. A 2-hr incuba neic (rat) lymphocytes (14). Moreover, we have reported tion of macrophages with MAF was sufficient to initiate some success in treating pulmonary tumor colonies in mice activation, providing that 46 hr were allowed to elapse be by iv. injection ofin vitro-activated macrophages (13). More fore tumor cells were added. The MAF activity was en recently, we have investigated in vitro activation of mouse hanced after heating the supernatant to 100?°.Control ex macrophage