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8490 J. Agric. Food Chem. 2010, 58, 8490–8494 DOI:10.1021/jf100598k Detection of Six Genetically Modified Maize Lines Using Optical Thin-Film Biosensor Chips ^ ?, ) ?, ) ?, ) #, ) SULAN BAI, JIE ZHANG, SHUCHENG LI, HAODONG CHEN, WILLIAM TERZAGHI, ? ? ? ? § XIN ZHANG, XIURONG CHI, JIN TIAN, HONGXIA LUO, WENSHENG HUANG, ,§ ,? YING CHEN,* AND YAOCHUAN ZHANG* ?College of Life Sciences, Capital Normal University, Beijing 100048, People’s Republic of China, ?Beijing Vocational College of Agriculture, Beijing 102442, People’s Republic of China, §Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100025, People’s Republic of China, #College of Life Sciences, Peking University, Beijing 100871, People’s Republic of China, and ^Department of Biology, Wilkes University, Wilkes-Barre, Pennsylvania 18766. ) Equal contribution to this work. As more and more genetically modified organisms (GMO) are commercialized, efficient and inexpen- sive assays are required for their quick detection. An event-specific detection strategy based on the unique and specific sequences of integration junctions is useful because of its high specificity. This study developed a system for detecting six GM maize lines (Bt11, Bt176, GA21, MON810, NK603, and T25) using optical silicon thin-film biosensor chips. Aldehyde-labeled probes were arrayed and covalently attached to a hydrazine-derivatized chip